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Use of protein-based biomarkers of exfoliated cervical cells for primary screening of cervical cancer

Title
Use of protein-based biomarkers of exfoliated cervical cells for primary screening of cervical cancer
Authors
Jin Y.Kim S.C.Kim H.J.Ju W.Kim Y.H.Kim H.-J.
Ewha Authors
김승철주웅김윤환
SCOPUS Author ID
김승철scopus; 주웅scopus; 김윤환scopus
Issue Date
2018
Journal Title
Archives of Pharmacal Research
ISSN
0253-6269JCR Link
Citation
Archives of Pharmacal Research vol. 41, no. 4, pp. 438 - 449
Keywords
Cervical cancerExfoliated cervical cellHuman papillomavirusPap testTumor-associated protein
Publisher
Pharmaceutical Society of Korea
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
There has been no attempt to apply protein-based markers of exfoliated cervical cells (ECCs) for primary screening of cervical cancer. In the present study, the levels of six tumor-associated protein [TAPs: Sialyl Lewis A (SLeA), Cancer Antigen 15-3 (CA 15-3), p53, heat shock protein (Hsp)70, Hsp27 and squamous cervical carcinoma antigen (SCCA)]and of two human papillomavirus (HPV) viral proteins (HPV16 E7 and HPV16 L1) of ECCs lysates were evaluated by enzyme-linked immunosorbent assays (ELISAs).The wells of 96-well plates were coated with the ECCs lysates from normal, cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III and cancer groups, and candidate proteins were detected by relevant antibodies. SLeA level decreased with increasing severity of lesions, whereas the levels of other candidate proteins increased. SLeA, HPV16 L1 and p53 levels appeared more useful for detecting cervical lesions than the other candidates. The combination of ELISA-SLeA and ELISA-HPV16 L1 could efficiently detect cervical lesions from normal. The combination of ELISA-SLeA and ELISA-p53 could powerfully discriminate cancer from normal with 91.3% sensitivity and 96.7% specificity. The protein levels of ECCs have great potential as biomarkers for primary screening of cervical cancer. © 2018, The Pharmaceutical Society of Korea.
DOI
10.1007/s12272-018-1015-5
Appears in Collections:
의과대학 > 의학과 > Journal papers
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