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Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution
- Title
- Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution
- Authors
- Baritugo, Kei-Anne; Kim, Hee Taek; David, Yokimiko; Khang, Tae Uk; Hyun, Sung Min; Kang, Kyoung Hee; Yu, Ju Hyun; Choi, Jong Hyun; Song, Jae Jun; Joo, Jeong Chan; Park, Si Jae
- Ewha Authors
- 박시재
- SCOPUS Author ID
- 박시재
- Issue Date
- 2018
- Journal Title
- MICROBIAL CELL FACTORIES
- ISSN
- 1475-2859
- Citation
- MICROBIAL CELL FACTORIES vol. 17
- Keywords
- Corynebacterium glutamicum; Co-utilization; Xylose; Gamma-aminobutyrate; Empty fruit bunch
- Publisher
- BMC
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Background: Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gamma-aminobutyrate (GABA), a versatile bulk chemical used in food and pharmaceutical industry, is also used as a key monomer for nylon 4. GABA can be biologically produced by decarboxylation of glutamate. Results: In this study, we examined high glutamate-producing Corynebacterium glutamicum strains as hosts for enhanced production of GABA from glucose and xylose as carbon sources. An Escherichia coli gadB mutant with a broad pH range of activity and E. coli xylAB genes were expressed under the control of a synthetic H36 promoter. When empty fruit bunch (EFB) solution was used as carbon source (45 g/L glucose and 5 g/L xylose), 12.54 +/- 0.07 g/L GABA was produced by recombinant C. glutamicum H36GD1852 expressing E. coli gadB mutant gene and xylAB genes. Batch fermentation of the same strain resulted in the production of 35.47 g/L of GABA when EFB solution was added to support 90 g/L glucose and 10 g/L xylose. Conclusions: This is the first report of GABA production by recombinant C. glutamicum strains from co-utilization of glucose and xylose from EFB solution. Recombinant C. glutamicum strains developed in this study should be useful for an efficient and sustainable production of GABA from lignocellulosic biomasses.
- DOI
- 10.1186/s12934-018-0977-9
- Appears in Collections:
- 공과대학 > 화공신소재공학과 > Journal papers
- Files in This Item:
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