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Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution

Title
Enhanced production of gamma-aminobutyrate (GABA) in recombinant Corynebacterium glutamicum strains from empty fruit bunch biosugar solution
Authors
Baritugo, Kei-AnneKim, Hee TaekDavid, YokimikoKhang, Tae UkHyun, Sung MinKang, Kyoung HeeYu, Ju HyunChoi, Jong HyunSong, Jae JunJoo, Jeong ChanPark, Si Jae
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2018
Journal Title
MICROBIAL CELL FACTORIES
ISSN
1475-2859JCR Link
Citation
MICROBIAL CELL FACTORIES vol. 17
Keywords
Corynebacterium glutamicumCo-utilizationXyloseGamma-aminobutyrateEmpty fruit bunch
Publisher
BMC
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Background: Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns on global warming and depletion of fossil fuel reserves. Microbial production of platform chemicals in biorefineries has been suggested to be a promising solution for these problems. Gamma-aminobutyrate (GABA), a versatile bulk chemical used in food and pharmaceutical industry, is also used as a key monomer for nylon 4. GABA can be biologically produced by decarboxylation of glutamate. Results: In this study, we examined high glutamate-producing Corynebacterium glutamicum strains as hosts for enhanced production of GABA from glucose and xylose as carbon sources. An Escherichia coli gadB mutant with a broad pH range of activity and E. coli xylAB genes were expressed under the control of a synthetic H36 promoter. When empty fruit bunch (EFB) solution was used as carbon source (45 g/L glucose and 5 g/L xylose), 12.54 +/- 0.07 g/L GABA was produced by recombinant C. glutamicum H36GD1852 expressing E. coli gadB mutant gene and xylAB genes. Batch fermentation of the same strain resulted in the production of 35.47 g/L of GABA when EFB solution was added to support 90 g/L glucose and 10 g/L xylose. Conclusions: This is the first report of GABA production by recombinant C. glutamicum strains from co-utilization of glucose and xylose from EFB solution. Recombinant C. glutamicum strains developed in this study should be useful for an efficient and sustainable production of GABA from lignocellulosic biomasses.
DOI
10.1186/s12934-018-0977-9
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엘텍공과대학 > 화학신소재공학전공 > Journal papers
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