Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이미애 | * |
dc.contributor.author | 정혜선 | * |
dc.date.accessioned | 2018-12-07T16:30:46Z | - |
dc.date.available | 2018-12-07T16:30:46Z | - |
dc.date.issued | 2017 | * |
dc.identifier.issn | 1081-5589 | * |
dc.identifier.other | OAK-20563 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/247421 | - |
dc.description.abstract | Rapid and accurate diagnosis of Clostridium difficile infection (CDI) is crucial for patient care, infection control, and efficient surveillance. We evaluated C. DIFF QUIK CHEK COMPLETE (QCC; TechLab), which detects glutamate dehydrogenase (GDH) antigen (QCC-Ag) and toxin A/B (QCC-Tox) simultaneously, and compared it to the laboratory diagnostics for CDI currently in use in a tertiary hospital setting with a high prevalence of CDI. QCC, RIDASCREEN C. difficile toxin A/B assay (Toxin EIA; R-Biopharm AG), chromID C. difficile agar (bioMérieux) culture (ChromID culture), and Xpert C. difficile PCR assay (Xpert PCR; Cepheid) were performed according to the manufacturers' instructions. Performances of the assays were compared against that of Xpert PCR as a reference. Of the 231 loose stool specimens, 83 (35.9%) were positive by Xpert PCR. The sensitivity, specificity, and positive and negative predictive values were 97.6%, 93.9%, 90.0%, and 98.6%, respectively, for QCC-Ag and 55.4%, 100%, 100%, and 80.0%, respectively, for QCC-Tox. The median threshold cycle values of the QCC-Tox(+) specimens were lower than those of the QCC-Tox(-) specimens. Results: of QCC as an initial screening test were confirmed in 81.0% (187/231) of samples; these specimens did not require further testing. QCC is a rapid, easy, and cost-effective method that would be a useful first-line screening assay for laboratory diagnosis of CDI in a tertiary hospital with a high prevalence of CDI. A two-step algorithm using QCC as an initial screening tool, followed by Xpert PCR as a confirmatory test, is a practical and cost-effective approach. Copyright © 2016 American Federation for Medical Research. | * |
dc.description.sponsorship | Ewha Womans University | * |
dc.language | English | * |
dc.publisher | BMJ Publishing Group | * |
dc.title | Evaluation of the performance of C. DIFF QUIK CHEK COMPLETE and its usefulness in a hospital setting with a high prevalence of Clostridium difficile infection | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 65 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 88 | * |
dc.relation.lastpage | 92 | * |
dc.relation.journaltitle | Journal of Investigative Medicine | * |
dc.identifier.doi | 10.1136/jim-2016-000231 | * |
dc.identifier.wosid | WOS:000398792400013 | * |
dc.identifier.scopusid | 2-s2.0-85035356890 | * |
dc.author.google | Chung H.-S. | * |
dc.author.google | Lee M. | * |
dc.contributor.scopusid | 이미애(7409114044) | * |
dc.contributor.scopusid | 정혜선(7404006436) | * |
dc.date.modifydate | 20240222163122 | * |