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Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts

Title
Low-Affinity Zinc Sensor Showing Fluorescence Responses with Minimal Artifacts
Authors
Yan X.Kim J.J.Jeong H.S.Moon Y.K.Cho Y.K.Ahn S.Jun S.B.Kim H.You Y.
Ewha Authors
유영민전상범
SCOPUS Author ID
유영민scopus; 전상범scopus
Issue Date
2017
Journal Title
Inorganic Chemistry
ISSN
0020-1669JCR Link
Citation
Inorganic Chemistry vol. 56, no. 8, pp. 4332 - 4346
Publisher
American Chemical Society
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
The study of the zinc biology requires molecular probes with proper zinc affinity. We developed a low-affinity zinc probe (HBO-ACR) based on an azacrown ether (ACR) and an 2-(2-hydroxyphenyl)benzoxazole (HBO) fluorophore. This probe design imposed positive charge in the vicinity of a zinc coordination center, which enabled fluorescence turn-on responses to high levels of zinc without being affected by the pH and the presence of other transition-metal ions. Steady-state and transient photophysical investigations suggested that such a high tolerance benefits from orchestrated actions of proton-induced nonradiative and zinc-induced radiative control. The zinc bioimaging utility of HBO-ACR has been fully demonstrated with the use of human pancreas epidermoid carcinoma, PANC-1 cells, and rodent hippocampal neurons from cultures and acute brain slices. The results obtained through our studies established the validity of incorporating positively charged ionophores for the creation of low-affinity probes for the visualization of biometals. © 2017 American Chemical Society.
DOI
10.1021/acs.inorgchem.6b02786
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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