View : 9 Download: 0

A metagenomic analysis provides a culture-independent pathogen detection for atopic dermatitis

Title
A metagenomic analysis provides a culture-independent pathogen detection for atopic dermatitis
Authors
Kim M.-H.Rho M.Choi J.-P.Choi H.-I.Park H.-K.Song W.-J.Min T.-K.Cho S.-H.Cho Y.-J.Kim Y.-K.Yang S.Pyun B.Y.
Ewha Authors
조영주김민혜
SCOPUS Author ID
조영주scopus; 김민혜scopus
Issue Date
2017
Journal Title
Allergy, Asthma and Immunology Research
ISSN
2092-7355JCR Link
Citation
Allergy, Asthma and Immunology Research vol. 9, no. 5, pp. 453 - 461
Keywords
Atopic dermatitis; Extracellular vesicle; Metagenomic analysis
Publisher
Korean Academy of Asthma, Allergy and Clinical Immunology
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
Purpose: Atopic dermatitis (AD) is an inflammatory skin disease, significantly affecting the quality of life. Using AD as a model system, we tested a successive identification of AD-associated microbes, followed by a culture-independent serum detection of the identified microbe. Methods: A total of 43 genomic DNA preparations from washing fluid of the cubital fossa of 6 healthy controls, skin lesions of 27 AD patients, 10 of which later received treatment (post-treatment), were subjected to high-throughput pyrosequencing on a Roche 454 GS-FLX platform. Results: Microbial diversity was decreased in AD, and was restored following treatment. AD was characterized by the domination of Staphylococcus, Pseudomonas, and Streptococcus, whereas Alcaligenaceae (f), Sediminibacterium, and Lactococcus were characteristic of healthy skin. An enzyme-linked immunosorbent assay (ELISA) showed that serum could be used as a source for the detection of Staphylococcus aureus extracellular vesicles (EVs). S. aureus EV-specific immunoglobulin G (IgG) and immunoglobulin E (IgE) were quantified in the serum. Conclusions: A metagenomic analysis together with a serum detection of pathogen-specific EVs provides a model for successive identification and diagnosis of pathogens of AD. © The Korean Academy of Asthma, Allergy and Clinical Immunology.
DOI
10.4168/aair.2017.9.5.453
Appears in Collections:
의과대학 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE