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Structural and Biochemical Characterization of the Curcumin-Reducing Activity of CurA from Vibrio vulnificus

Title
Structural and Biochemical Characterization of the Curcumin-Reducing Activity of CurA from Vibrio vulnificus
Authors
Park, Soo-BongBae, Da-WoonClavio, Nina Abigail B.Zhao, LeiJeong, Chang-SookChoi, Bo MeeMacalino, Stephani Joy Y.Cha, Hee-JeongPark, Jin-ByungLee, Jun HyuckNam, Sang-JipChoi, SunKim, Min-KyuCha, Sun-Shin
Ewha Authors
최선박진병남상집차선신
SCOPUS Author ID
최선scopus; 박진병scopus; 남상집scopus; 차선신scopus
Issue Date
2018
Journal Title
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN
0021-8561JCR Link

1520-5118JCR Link
Citation
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 66, no. 40, pp. 10608 - 10616
Keywords
curcumin-reducing enzyme; crystal structure of apo VvCurA; crystal structure of the VvCurA/NADPH complex; in silico model of the VvCurA/NADPH/curcumin complex; enzyme mechanism
Publisher
AMER CHEMICAL SOC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Curcumin is a yellow-colored ingredient in dietary spice turmeric (Curcuma longa Linn). This nontoxic polyphenol has antitumor, anti-inflammatory, apoptotic, and antioxidant activities. The ingested curcumin is reduced to multihydrated forms with more potent therapeutic potentials by the curcumin reductase (CurA) from commensal Escherichia coli. In this study, we demonstrated that Vibrio vulnificus CurA (VvCurA) with 87% sequence similarity to the E. coli CurA exhibits the curcumin-reducing activity through spectrophotometric detection of NADPH oxidation and high performance liquid chromatographic analysis of curcumin consumption and product generation. Afterward, we determined the crystal structures of VvCurA and the VvCurA/NADPH complex, and made the in silico model of the VvCurA/NADPH/curcumin ternary complex through induced fit docking. Based on structural information, active site residues that play critical roles in catalysis have been identified and characterized by mutational and kinetic studies, leading us to propose the reaction mechanism of CurA.
DOI
10.1021/acs.jafc.8b03647
Appears in Collections:
약학대학 > 약학과 > Journal papers
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