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일반대학원
바이오융합과학과
Journal papers
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Construction of a scFv library with synthetic, non-combinatorial CDR diversity
Title
Construction of a scFv library with synthetic, non-combinatorial CDR diversity
Authors
Bai X.
;
Shim H.
Ewha Authors
심현보
SCOPUS Author ID
심현보
Issue Date
2017
Journal Title
Methods in Molecular Biology
ISSN
1064-3745
Citation
Methods in Molecular Biology vol. 1575, pp. 15 - 29
Keywords
Antibody library
;
Non-combinatorial CDR diversity
;
Phage display
;
scFv
;
Synthetic antibody library
;
Synthetic CDR diversity
Publisher
Humana Press Inc.
Indexed
SCOPUS
Document Type
Book Chapter
Abstract
Many large synthetic antibody libraries have been designed, constructed, and successfully generated high-quality antibodies suitable for various demanding applications. While synthetic antibody libraries have many advantages such as optimized framework sequences and a broader sequence landscape than natural antibodies, their sequence diversities typically are generated by random combinatorial synthetic processes which cause the incorporation of many undesired CDR sequences. Here, we describe the construction of a synthetic scFv library using oligonucleotide mixtures that contain predefined, non-combinatorially synthesized CDR sequences. Each CDR is first inserted to a master scFv framework sequence and the resulting single-CDR libraries are subjected to a round of proofread panning. The proofread CDR sequences are assembled to produce the final scFv library with six diversified CDRs. © Springer Science+Business Media LLC 2017.
DOI
10.1007/978-1-4939-6857-2_2
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