View : 88 Download: 0

Construction of a scFv library with synthetic, non-combinatorial CDR diversity

Title
Construction of a scFv library with synthetic, non-combinatorial CDR diversity
Authors
Bai X.Shim H.
Ewha Authors
심현보
SCOPUS Author ID
심현보scopus
Issue Date
2017
Journal Title
Methods in Molecular Biology
ISSN
1064-3745JCR Link
Citation
Methods in Molecular Biology vol. 1575, pp. 15 - 29
Keywords
Antibody libraryNon-combinatorial CDR diversityPhage displayscFvSynthetic antibody librarySynthetic CDR diversity
Publisher
Humana Press Inc.
Indexed
SCOPUS scopus
Document Type
Book Chapter
Abstract
Many large synthetic antibody libraries have been designed, constructed, and successfully generated high-quality antibodies suitable for various demanding applications. While synthetic antibody libraries have many advantages such as optimized framework sequences and a broader sequence landscape than natural antibodies, their sequence diversities typically are generated by random combinatorial synthetic processes which cause the incorporation of many undesired CDR sequences. Here, we describe the construction of a synthetic scFv library using oligonucleotide mixtures that contain predefined, non-combinatorially synthesized CDR sequences. Each CDR is first inserted to a master scFv framework sequence and the resulting single-CDR libraries are subjected to a round of proofread panning. The proofread CDR sequences are assembled to produce the final scFv library with six diversified CDRs. © Springer Science+Business Media LLC 2017.
DOI
10.1007/978-1-4939-6857-2_2
Appears in Collections:
일반대학원 > 바이오융합과학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE