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Isolation and characterization of vaccine candidate genes including CSP and MSP1 in Plasmodium yoelii

Title
Isolation and characterization of vaccine candidate genes including CSP and MSP1 in Plasmodium yoelii
Authors
Kim S.-H.Bae Y.-A.Seoh J.-Y.Yang H.-J.
Ewha Authors
서주영양현종
SCOPUS Author ID
서주영scopus; 양현종scopus
Issue Date
2017
Journal Title
Korean Journal of Parasitology
ISSN
0023-4001JCR Link
Citation
Korean Journal of Parasitology vol. 55, no. 3, pp. 255 - 265
Keywords
CSPDBPMSP1Plasmodium yoeliiProtective immunityRodent malaria modelVaccine candidate
Publisher
Korean Journal of Parasitology
Indexed
SCOPUS; KCI; SCIE scopus
Document Type
Article
Abstract
Malaria is an infectious disease affecting humans, which is transmitted by the bite of Anopheles mosquitoes harboring sporozoites of parasitic protozoans belonging to the genus Plasmodium. Despite past achievements to control the protozoan disease, malaria still remains a significant health threat up to now. In this study, we cloned and characterized the full-unit Plasmodium yoelii genes encoding merozoite surface protein 1 (MSP1), circumsporozoite protein (CSP), and Duffy-binding protein (DBP), each of which can be applied for investigations to obtain potent protective vaccines in the rodent malaria model, due to their specific expression patterns during the parasite life cycle. Recombinant fragments corresponding to the middle and C-terminal regions of PyMSP1 and PyCSP, respectively, displayed strong reactivity against P. yoelii-infected mice sera. Specific native antigens invoking strong humoral immune response during the primary and secondary infections of P. yoelii were also abundantly detected in experimental ICR mice. The low or negligible parasitemia observed in the secondary infected mice was likely to result from the neutralizing action of the protective antibodies. Identification of these antigenic proteins might provide the necessary information and means to characterize additional vaccine candidate antigens, selected solely on their ability to produce the protective antibodies. © 2017, Korean Society for Parasitology and Tropical Medicine.
DOI
10.3347/kjp.2017.55.3.255
Appears in Collections:
의과대학 > 의학과 > Journal papers
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