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자연과학대학
화학·나노과학전공
Journal papers
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Cholic acid attenuates ER stress-induced cell death in coxsackievirus-B3 infection
Title
Cholic acid attenuates ER stress-induced cell death in coxsackievirus-B3 infection
Authors
Han J.-Y.
;
Jeong H.I.
;
Park C.-W.
;
Yoon J.
;
Ko J.
;
Nam S.-J.
;
Lim B.-K.
Ewha Authors
남상집
SCOPUS Author ID
남상집
Issue Date
2018
Journal Title
Journal of Microbiology and Biotechnology
ISSN
1017-7825
Citation
Journal of Microbiology and Biotechnology vol. 28, no. 1, pp. 109 - 114
Keywords
Cholic acid
;
Coxsackievirus B3
;
ER stress
;
Myocarditis
;
Proliferation
Publisher
Korean Society for Microbiology and Biotechnology
Indexed
SCIE; SCOPUS; KCI
Document Type
Article
Abstract
Coxsackievirus Type B3 (CVB3) is an enterovirus that belongs to the Picornaviridae and causes various diseases such as myocarditis and hand-foot-mouth disease. However, an effective antiviral drug is still not developed. In this study, we looked for potential inhibitors of CVB3 replication by examining the survival of CVB3-infected HeLa cells. We detected an antiviral effect by cholic acid and identified it as a candidate inhibitor of CVB3 replication. Cholic acid circulates in the liver and intestines, and it helps the digestion and absorption of lipids in the small intestine. HeLa cells were cultured in 12-well plates and treated with cholic acid (1 and 10 µg/ml) and 106 PFU/ml of CVB3. After 16 h post-infection, the cells were lysed and subjected to western blot analysis and RT-PCR. The production of the viral capsid protein VP1 was dramatically decreased, and translation initiation factor eIF4G1 cleavage was significantly inhibited by treatment with 10 µg/ml cholic acid. Moreover, cholic acid inhibited ERK signaling in CVB3-infected HeLa cells. RT-PCR showed that the amounts of the CVB3 RNA genome and mRNA for the ER stress-related transcription factor ATF4 were significantly reduced. These results showed that cholic acid strongly reduced ER stress and CVB3 proliferation. This compound can be developed as a safe natural therapeutic agent for enterovirus infections. © 2018 by The Korean Society for Microbiology and Biotechnology.
DOI
10.4014/jmb.1708.08009
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