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Repression of adenosine triphosphate-binding cassette transporter ABCG2 by estrogen increases intracellular glutathione in brain endothelial cells following ischemic reperfusion injury

Title
Repression of adenosine triphosphate-binding cassette transporter ABCG2 by estrogen increases intracellular glutathione in brain endothelial cells following ischemic reperfusion injury
Authors
Shin, Jin A.Jeong, Sae ImKim, Hye WonJang, GyeonghuiRyu, Dong-RyeolAhn, Young-HoChoi, Ji HaChoi, Youn-HeePark, Eun-Mi
Ewha Authors
박은미류동열최윤희최지하안영호
SCOPUS Author ID
박은미scopus; 류동열scopus; 최윤희scopus; 최지하scopus; 안영호scopus
Issue Date
2018
Journal Title
NEUROBIOLOGY OF AGING
ISSN
0197-4580JCR Link

1558-1497JCR Link
Citation
NEUROBIOLOGY OF AGING vol. 66, pp. 138 - 148
Keywords
ABCG2Brain endothelial cellEstrogenGlutathioneIschemic strokeNeuroprotection
Publisher
ELSEVIER SCIENCE INC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
The adenosine triphosphate-binding cassette efflux transporter ABCG2, which is located in the blood-brain barrier limits the entry of endogenous compounds and xenobiotics into the brain, and its expression and activity are regulated by estrogen. This study was aimed to define the role of ABCG2 in estrogen-mediated neuroprotection against ischemic injury. ABCG2 protein levels before and after ischemic stroke were increased in the brain of female mice by ovariectomy, which were reversed by estrogen replacement. In brain endothelial cell line bEnd. 3, estrogen reduced the basal ABCG2 protein level and efflux activity and protected cells from ischemic injury without inducing ABCG2 expression. When bEnd. 3 cells were transfected with ABCG2 small interfering RNA, ischemia-induced cell death was reduced, and the intracellular concentration of glutathione, an antioxidant that is transported by ABCG2, was increased. In addition, after ischemic stroke in ovariectomized mice, estrogen prevented the reduction of intracellular glutathione level in brain microvessels. These data suggested that the suppression of ABCG2 by estrogen is involved in neuroprotection against ischemic injury by increasing intracellular glutathione, and that the modulation of ABCG2 activity offers a therapeutic target for brain diseases in estrogen-deficient aged women. (C) 2018 Elsevier Inc. All rights reserved.
DOI
10.1016/j.neurobiolaging.2018.02.020
Appears in Collections:
의과대학 > 의학과 > Journal papers
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