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dc.contributor.author서주영-
dc.date.accessioned2018-11-21T16:30:24Z-
dc.date.available2018-11-21T16:30:24Z-
dc.date.issued2018-
dc.identifier.issn0385-5600-
dc.identifier.otherOAK-22358-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/246734-
dc.description.abstractPrevious studies have examined various immune evasion strategies of human cytomegalovirus (HCMV) to gain understanding of its pathogenesis. Although the mechanism that underlies immunocyte destruction near HCMV-infected lesions has yet to be established, it is here shown that substances produced by HCMV-infected cells induce death in several types of immunocytes, but not in fibroblasts or astrocytomas. These substances contain HCMV proteins and were termed HCMV-associated insoluble substance (HCMVAIS). The mechanism by which HCMVAIS induces cell death was characterized to improve understanding the death of immunocytes near HCMV-infected lesions. HCMVAIS were found to trigger production of intracellular nicotinamide adenine dinucleotide phosphate oxidase-derived reactive oxygen species (ROS), resulting in cell death, this effect being reversed following treatment with ROS inhibitors. Cell death was not induced in splenocytes from NOX-2 knockout mice. It was hypothesized that DNA damage induced by oxidative stress initiates poly ADP-ribose polymerase-1 (PARP-1)-mediated cell death, or parthanatos. HCMVAIS-induced cell death is accompanied by PARP-1 activation in a caspase-independent manner, nuclear translocation of apoptosis-inducing factor (AIF), and DNA fragmentation, which are typical features of parthanatos. Treatment with an AIF inhibitor decreased the rate of HCMVAIS-induced cell death, this being confirmed by hematoxylin and eosin staining; cell death in most HCMV-positive foci in serial section samples of a large intestine with HCMV infection was TUNEL-positive, cleaved caspase 3-negative and CD45-positive. Taken together, these data suggest that HCMV inhibits local immune responses via direct killing of immunocytes near HCMV-infected cells through ROS-induced parthanatos by HCMVAIS. © 2018 The Authors. Microbiology and Immunology published by The Societies and John Wiley & Sons Australia, Ltd-
dc.languageEnglish-
dc.publisherBlackwell Publishing Asia-
dc.subjecthuman cytomegalovirus-
dc.subjectparthanatos-
dc.subjectreactive oxygen species-
dc.titleReactive oxygen species-induced parthanatos of immunocytes by human cytomegalovirus-associated substance-
dc.typeArticle-
dc.relation.issue4-
dc.relation.volume62-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage229-
dc.relation.lastpage242-
dc.relation.journaltitleMicrobiology and Immunology-
dc.identifier.doi10.1111/1348-0421.12575-
dc.identifier.wosidWOS:000431009100003-
dc.identifier.scopusid2-s2.0-85042592427-
dc.author.googleKim J.H.-
dc.author.googleKim J.-
dc.author.googleRoh J.-
dc.author.googlePark C.-S.-
dc.author.googleSeoh J.-Y.-
dc.author.googleHwang E.-S.-
dc.contributor.scopusid서주영(6603709174)-
dc.date.modifydate20181121115107-
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의과대학 > 의학과 > Journal papers
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