Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 서주영 | - |
dc.date.accessioned | 2018-11-21T16:30:24Z | - |
dc.date.available | 2018-11-21T16:30:24Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0385-5600 | - |
dc.identifier.other | OAK-22358 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/246734 | - |
dc.description.abstract | Previous studies have examined various immune evasion strategies of human cytomegalovirus (HCMV) to gain understanding of its pathogenesis. Although the mechanism that underlies immunocyte destruction near HCMV-infected lesions has yet to be established, it is here shown that substances produced by HCMV-infected cells induce death in several types of immunocytes, but not in fibroblasts or astrocytomas. These substances contain HCMV proteins and were termed HCMV-associated insoluble substance (HCMVAIS). The mechanism by which HCMVAIS induces cell death was characterized to improve understanding the death of immunocytes near HCMV-infected lesions. HCMVAIS were found to trigger production of intracellular nicotinamide adenine dinucleotide phosphate oxidase-derived reactive oxygen species (ROS), resulting in cell death, this effect being reversed following treatment with ROS inhibitors. Cell death was not induced in splenocytes from NOX-2 knockout mice. It was hypothesized that DNA damage induced by oxidative stress initiates poly ADP-ribose polymerase-1 (PARP-1)-mediated cell death, or parthanatos. HCMVAIS-induced cell death is accompanied by PARP-1 activation in a caspase-independent manner, nuclear translocation of apoptosis-inducing factor (AIF), and DNA fragmentation, which are typical features of parthanatos. Treatment with an AIF inhibitor decreased the rate of HCMVAIS-induced cell death, this being confirmed by hematoxylin and eosin staining; cell death in most HCMV-positive foci in serial section samples of a large intestine with HCMV infection was TUNEL-positive, cleaved caspase 3-negative and CD45-positive. Taken together, these data suggest that HCMV inhibits local immune responses via direct killing of immunocytes near HCMV-infected cells through ROS-induced parthanatos by HCMVAIS. © 2018 The Authors. Microbiology and Immunology published by The Societies and John Wiley & Sons Australia, Ltd | - |
dc.language | English | - |
dc.publisher | Blackwell Publishing Asia | - |
dc.subject | human cytomegalovirus | - |
dc.subject | parthanatos | - |
dc.subject | reactive oxygen species | - |
dc.title | Reactive oxygen species-induced parthanatos of immunocytes by human cytomegalovirus-associated substance | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 62 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 229 | - |
dc.relation.lastpage | 242 | - |
dc.relation.journaltitle | Microbiology and Immunology | - |
dc.identifier.doi | 10.1111/1348-0421.12575 | - |
dc.identifier.wosid | WOS:000431009100003 | - |
dc.identifier.scopusid | 2-s2.0-85042592427 | - |
dc.author.google | Kim J.H. | - |
dc.author.google | Kim J. | - |
dc.author.google | Roh J. | - |
dc.author.google | Park C.-S. | - |
dc.author.google | Seoh J.-Y. | - |
dc.author.google | Hwang E.-S. | - |
dc.contributor.scopusid | 서주영(6603709174;57209001625) | - |
dc.date.modifydate | 20230210140937 | - |