Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 유영민 | * |
dc.contributor.author | 손아정 | * |
dc.date.accessioned | 2018-11-21T16:30:18Z | - |
dc.date.available | 2018-11-21T16:30:18Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 0956-5663 | * |
dc.identifier.other | OAK-23029 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/246703 | - |
dc.description.abstract | We have developed a quantum dot aptasensor (QD-aptasensor) and its accompanying portable analyzer for the detection of di-2-ethylhexyl phthalate (DEHP). This sensor is based on a newly screened aptamer (60-mer) via SELEX and shows a binding affinity of 213 nmol/L with DEHP. The 60-mer aptamer together with its three shorter truncated aptamers (45, 28, and 22-mer) as well as three different DNA probes (12, 9, and 13-mer) were further investigated to form the best combination for the QD-aptasensor. Using a 22-mer-truncated aptamer and a 12-mer DNA probe combination, the QD-aptasensor demonstrated excellent DEHP sensitivity with an LOQ = 0.5 pg/mL as well as good selectivity in the presence of other phthalate analogs. The binding between the truncated aptamers and DEHP was also characterized. Finally, a QD-aptasensor-based portable analyzer was also developed, and its equivalence to the laboratory protocol was established with a correlation coefficient r = 0.86 for DEHP concentrations ranging from 0.0005 to 100 ng/mL. © 2018 Elsevier B.V. | * |
dc.language | English | * |
dc.publisher | Elsevier Ltd | * |
dc.subject | Aptamer | * |
dc.subject | Di-2-ethylhexyl phthalate | * |
dc.subject | Portable analyzer | * |
dc.subject | Quantum dot aptasensor | * |
dc.title | Development of quantum dot aptasensor and its portable analyzer for the detection of di-2-ethylhexyl phthalate | * |
dc.type | Article | * |
dc.relation.volume | 121 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1 | * |
dc.relation.lastpage | 9 | * |
dc.relation.journaltitle | Biosensors and Bioelectronics | * |
dc.identifier.doi | 10.1016/j.bios.2018.08.065 | * |
dc.identifier.wosid | WOS:000447819700001 | * |
dc.identifier.scopusid | 2-s2.0-85052888818 | * |
dc.author.google | Lim H.J. | * |
dc.author.google | Kim A.-R. | * |
dc.author.google | Yoon M.-Y. | * |
dc.author.google | You Y. | * |
dc.author.google | Chua B. | * |
dc.author.google | Son A. | * |
dc.contributor.scopusid | 유영민(24759463600) | * |
dc.contributor.scopusid | 손아정(23487252600) | * |
dc.date.modifydate | 20240422132144 | * |