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Development of quantum dot aptasensor and its portable analyzer for the detection of di-2-ethylhexyl phthalate

Title
Development of quantum dot aptasensor and its portable analyzer for the detection of di-2-ethylhexyl phthalate
Authors
Lim H.J.Kim A.-R.Yoon M.-Y.You Y.Chua B.Son A.
Ewha Authors
유영민손아정
SCOPUS Author ID
유영민scopus; 손아정scopus
Issue Date
2018
Journal Title
Biosensors and Bioelectronics
ISSN
0956-5663JCR Link
Citation
Biosensors and Bioelectronics vol. 121, pp. 1 - 9
Keywords
AptamerDi-2-ethylhexyl phthalatePortable analyzerQuantum dot aptasensor
Publisher
Elsevier Ltd
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
We have developed a quantum dot aptasensor (QD-aptasensor) and its accompanying portable analyzer for the detection of di-2-ethylhexyl phthalate (DEHP). This sensor is based on a newly screened aptamer (60-mer) via SELEX and shows a binding affinity of 213 nmol/L with DEHP. The 60-mer aptamer together with its three shorter truncated aptamers (45, 28, and 22-mer) as well as three different DNA probes (12, 9, and 13-mer) were further investigated to form the best combination for the QD-aptasensor. Using a 22-mer-truncated aptamer and a 12-mer DNA probe combination, the QD-aptasensor demonstrated excellent DEHP sensitivity with an LOQ = 0.5 pg/mL as well as good selectivity in the presence of other phthalate analogs. The binding between the truncated aptamers and DEHP was also characterized. Finally, a QD-aptasensor-based portable analyzer was also developed, and its equivalence to the laboratory protocol was established with a correlation coefficient r = 0.86 for DEHP concentrations ranging from 0.0005 to 100 ng/mL. © 2018 Elsevier B.V.
DOI
10.1016/j.bios.2018.08.065
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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