Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이지희 | * |
dc.contributor.author | 최윤희 | * |
dc.contributor.author | 박상희 | * |
dc.contributor.author | 박현주 | * |
dc.date.accessioned | 2018-11-16T16:30:12Z | - |
dc.date.available | 2018-11-16T16:30:12Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 1015-8987 | * |
dc.identifier.issn | 1421-9778 | * |
dc.identifier.other | OAK-23476 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/246574 | - |
dc.description.abstract | Background/Aims: Glioblastoma multiforme (GBM) is the most common primary brain tumor in adults. The defining characteristics of GBM are diffuse infiltration of tumor cells into normal brain parenchyma, rapid growth, a high degree of infiltration of microglia and macrophages, and the presence of necrosis. Microglia/macrophages are frequently found in gliomas and they extensively infiltrate GBM tissue, up to 30% of total tumor mass. However, little is known about the effect of necrotic cells (NCs) on microglia infiltration in GBM and the tumor-infiltrating microglia-induced factors in GBMs. Methods: In this study, to address whether necrosis or necrosis-exposed GBM cells affect the degree of microglia/macrophage infiltration, migration and invasion/infiltration assays were performed. Culture supernatants and nuclear extracts of CRT-MG cells treated or untreated with necrotic cells were analyzed using a chemokine array and electrophoretic mobility shift assay, respectively. Results: The presence of NCs promoted the migration/infiltration of microglia, and GBM cell line CRT-MG cells exposed to NCs further enhanced the migration and infiltration of HM06 microglial cells. Treatment with NCs induced mRNA and protein expression of chemokines such as Monocyte Chemoattractant Protein-1 (CCL2/MCP-1) and Macrophage Inflammatory Protein-3 alpha (CCL20/MIP-3 alpha) in CRT MG cells. In particular, CCL2/MCP-1 and CCL20/MIP-3 alpha were significantly increased in NC-treated CRT-MG cells. NCs induced DNA binding of the transcription factors Nuclear Factor (NF)-kappa B and Activator Protein 1 (AP-1) to the CCL2/MCP-1 and CCL20/MIP-3 alpha promoters, leading to increased CCL2/MCP-1 and CCL20/MIP-3 alpha mRNA and protein expression in CRT MG cells. Conclusion: These results provide evidence that NCs induce the expression of CCL2/MCP-1 and CCL20/MIP-3 alpha in glioblastoma cells through activation of NF-kappa B and AP-1 and facilitate the infiltration of microglia into tumor tissues. (C) 2018 The Author(s) Published by S Karger AG, Basel | * |
dc.language | English | * |
dc.publisher | KARGER | * |
dc.subject | Necrosis | * |
dc.subject | Glioblastoma | * |
dc.subject | Microglia | * |
dc.subject | Migration | * |
dc.subject | Infiltration | * |
dc.title | MCP-1 and MIP-3 alpha Secreted from Necrotic Cell-Treated Glioblastoma Cells Promote Migration/Infiltration of Microglia | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 48 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1332 | * |
dc.relation.lastpage | 1346 | * |
dc.relation.journaltitle | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | * |
dc.identifier.doi | 10.1159/000492092 | * |
dc.identifier.wosid | WOS:000443612500037 | * |
dc.identifier.scopusid | 2-s2.0-85052486649 | * |
dc.author.google | Jung, Yieun | * |
dc.author.google | Ahn, So-Hee | * |
dc.author.google | Park, Hyunju | * |
dc.author.google | Park, Sang Hui | * |
dc.author.google | Choi, Kyungsun | * |
dc.author.google | Choi, Chulhee | * |
dc.author.google | Kang, Jihee Lee | * |
dc.author.google | Choi, Youn-Hee | * |
dc.contributor.scopusid | 이지희(7404517577) | * |
dc.contributor.scopusid | 최윤희(7404776849) | * |
dc.contributor.scopusid | 최윤희(57190749692;58492359100) | * |
dc.contributor.scopusid | 박상희(12041890800) | * |
dc.contributor.scopusid | 박현주(55821533400) | * |
dc.date.modifydate | 20240220112152 | * |