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The implications of fragmented genomic DNA size range on the hybridization efficiency in NanoGene assay

Title
The implications of fragmented genomic DNA size range on the hybridization efficiency in NanoGene assay
Authors
Wang X.Chua B.Son A.
Ewha Authors
손아정
SCOPUS Author ID
손아정scopus
Issue Date
2018
Journal Title
Sensors (Switzerland)
ISSN
1424-8220JCR Link
Citation
vol. 18, no. 8
Keywords
DNA fragmentationHybridization efficiencyMagnetic beadsOptimum sizeQuantification capabilityQuantum dots
Publisher
MDPI AG
Indexed
SCIE; SCOPUS scopus
Abstract
DNA hybridization-based assays are well known for their ability to detect and quantify specific bacteria. Assays that employ DNA hybridization include a NanoGene assay, fluorescence in situ hybridization, and microarrays. Involved in DNA hybridization, fragmentation of genomic DNA (gDNA) is necessary to increase the accessibility of the probe DNA to the target gDNA. However, there has been no thorough and systematic characterization of different fragmented gDNA sizes and their effects on hybridization efficiency. An optimum fragmented size range of gDNA for the NanoGene assay is hypothesized in this study. Bacterial gDNA is fragmented via sonication into different size ranges prior to the NanoGene assay. The optimum size range of gDNA is determined via the comparison of respective hybridization efficiencies (in the form of quantification capabilities). Different incubation durations are also investigated. Finally, the quantification capability of the fragmented (at optimum size range) and unfragmented gDNA is compared. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
DOI
10.3390/s18082646
Appears in Collections:
엘텍공과대학 > 환경공학전공 > Journal papers
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