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dc.contributor.author김길현-
dc.date.accessioned2018-06-09T08:13:58Z-
dc.date.available2018-06-09T08:13:58Z-
dc.date.issued1996-
dc.identifier.issn0161-5890-
dc.identifier.otherOAK-12483-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/244772-
dc.description.abstractCytotoxic T lymphocytes recognize antigenic peptides in association with major histocompatibility complex class I proteins. Although a large set of class I binding peptides has been described, it is not yet easy to search for potentially antigenic peptides without synthesis of a panel of peptides, and subsequent binding assays. In order to predict HLA-A2.1-restricted antigenic epitopes, a computer model of the HLA-A2.1 molecule was established using X-ray crystallography data. In this model nonameric peptide sequences were aligned. In a molecular dynamics (MD) simulation with two sets of peptides known to be presented by HLA-A2.1, it was important to know the anchor amino acid residue preference and the distance between the anchor residues. We show here that the peptides bound to the HLA-A2.1 model structure possess a side chain of C-terminal anchor residue oriented into the binding groove with different distances between the two anchor residues from 15 to 21Å. We also synthesized a set of nonamer peptides containing amino acid sequences of Hepatitis B virus protein that were selected on the basis of previously described HLA-A2.1 specific motifs. When results obtained from the MD simulation were compared with functional binding assays using the TAP-deficient cell line T2, it was evident that the MD simulation method improves prediction of the HLA-A2.1 binding epitope sequence. These results suggest that this approach can provide a way to predict peptide epitopes and search for antigenic regions in sequences in a variety of antigens without screening a large number of synthetic peptides.-
dc.languageEnglish-
dc.titleSelection of peptides that bind to the HLA-A2.1 molecule by molecular modelling-
dc.typeArticle-
dc.relation.issue2-
dc.relation.volume33-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage221-
dc.relation.lastpage230-
dc.relation.journaltitleMolecular Immunology-
dc.identifier.doi10.1016/0161-5890(95)00065-8-
dc.identifier.scopusid2-s2.0-0029926327-
dc.author.googleLim J.-S.-
dc.author.googleKim S.-
dc.author.googleLee H.G.-
dc.author.googleLee K.-Y.-
dc.author.googleKwon T.-J.-
dc.author.googleKim K.-
dc.contributor.scopusid김길현(56092131700)-
dc.date.modifydate20211210152111-
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자연과학대학 > 생명과학전공 > Journal papers
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