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Immunolocalization of the 150 kDa protein in cyst fluid of Taenia solium metacestodes.

Title
Immunolocalization of the 150 kDa protein in cyst fluid of Taenia solium metacestodes.
Authors
Yang H.J.Chung Y.B.
Ewha Authors
양현종
SCOPUS Author ID
양현종scopus
Issue Date
2004
Journal Title
The Korean journal of parasitology
ISSN
0023-4001JCR Link
Citation
The Korean journal of parasitology vol. 42, no. 2, pp. 81 - 84
Indexed
SCIE; SCOPUS; KCI scopus
Document Type
Article
Abstract
The 150 kDa protein of cyst fluid (CF) of Taenia solium metacestodes was purified by ammonium sulfate fractionation and Superose 6 HR gel filtration chromatography. The purified protein consisted of three subunits (15, 10 and 7 kDa proteins), which were analyzed with the use of a 7.5-15% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Immunofluorescence study was carried out by using immunize specific polyclonal antibody. Positive reactions were noticed at bladder walls, calcareous corpuscles, granules of cyst fluid and some host tissue surrounding the bladder wall of the metacestodes. These results suggest that the 150 kDa protein was secreted into host tissues, inducing immune responses in the host, and it may play important roles in the cellular physiology of the parasites.
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의과대학 > 의학과 > Journal papers
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