Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 하종식 | * |
dc.contributor.author | 이지희 | * |
dc.date.accessioned | 2018-06-02T08:15:04Z | - |
dc.date.available | 2018-06-02T08:15:04Z | - |
dc.date.issued | 1998 | * |
dc.identifier.issn | 1226-4512 | * |
dc.identifier.other | OAK-17070 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/244379 | - |
dc.description.abstract | Platelet-activating factor (PAF) enhanced interleukin-1 (IL-1) activity by the interaction with a specific receptor in rat alveolar macrophages. In this study, we investigated the role of endogenous arachidonate metabolites and intracellular calcium mobilization in the PAF-induced IL-1 activity. Alveolar macrophages were preincubated with 5-lipoxygenase and cyclooxygenase inhibitors 30 min before the addition of PAF and lipopolysaccharide (LPS). After 24h culture, IL-1 activity was measured in the supernate of sample using the thymocyte proliferation assay. Inhibition of 5-lipoxyenase by nordihydroguaiaretic acid and AA-861 completely blocked the PAF-induced enhancement of IL-1 activity with IC50 of 2 μM and 5 μM, respectively. In contrast, the inhibition of cyclooxygenae pathway by indomethacin and ibuprofen resulted in the potentiation in PAF-induced IL-1 activity with maximal effect at 1 μM and 5 μM, respectively. In addition, leukotriene B4 and prostaglandin E2 production were observed in PAF-stimulated alveolar macrophage culture. As could be expected, 5-lipoxygenase and cyclooxygenase inhibitors abolished PAF-stimulated leukotriene B4 and prostaglandin E2 production, respectively. The effects of PAF on intracellular calcium mobilization in alveolar macrophages were evaluated using the calcium- sensitive dye fura-2 at the single cell level. PAF at any dose between 10- 16 and 10-8 M did not increase intracellular calcium. Furthermore, there was no effective change of intracellular level when PAF was added to alveolar macrophages in the presence of LPS or LPS+LTB4, and 4, 24, and 48h after treatment of these stimulants. Together, the results indicate that IL-1 activity induced by PAF is differently regulated through subsequent induction of endogenous 5-lipoxygenase and cyclooxygenase pathways, but not dependent on calcium signalling pathway. | * |
dc.language | English | * |
dc.title | Study on the action of PAF on IL-1 modulation in alveolar macrophages: Involvement of endogenous arachidonate metabolites and intracellular Ca++ mobilization | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 2 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 241 | * |
dc.relation.lastpage | 249 | * |
dc.relation.journaltitle | Korean Journal of Physiology and Pharmacology | * |
dc.identifier.scopusid | 2-s2.0-0031781060 | * |
dc.author.google | Lee J. | * |
dc.author.google | Kim W.-K. | * |
dc.author.google | Hah J.-S. | * |
dc.contributor.scopusid | 하종식(7003942216) | * |
dc.contributor.scopusid | 이지희(7404517577) | * |
dc.date.modifydate | 20240116125728 | * |