Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이경은 | * |
dc.date.accessioned | 2018-06-02T08:14:58Z | - |
dc.date.available | 2018-06-02T08:14:58Z | - |
dc.date.issued | 1999 | * |
dc.identifier.issn | 1226-4512 | * |
dc.identifier.other | OAK-17143 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/244341 | - |
dc.description.abstract | Intracellular accumulation of bile acids in the hepatocytes during cholestasis is thought to be pathogenic in cholestatic liver injury. Due to the detergent-like effect of the hydrophobic bile acids, hepatocellular injury has been attributed to direct membrane damage. However histological findings of cholestatic liver diseases suggest apoptosis can be a mechanism of cell death during cholestatic liver diseases instead of necrosis. To determine the pattern of hepatocellular toxicity induced by bile acid, we incubated primary cultured rat hepatocytes with a hydrophobic bile acid, Glycochenodeoxycholate (GCDC), up to 5 hours. After 5 hours incubation with 400 μM GCDC, lactate dehydrogenase released significantly. Cell viability, quantitated in propidium iodide stained cells concomitant with fluoresceindiacetate was decreased time- and dose-dependently. Most nuclei with condensed chromatin and shrunk cytoplasm were heavily labelled time- and dose-dependently by a positive TUNEL reaction. These findings suggest that both apoptosis and necrosis are involved in hepatocytes injury caused by GCDC. | * |
dc.language | English | * |
dc.title | Glycochenodeoxycholic acid induces cell death in primary cultured rat hepatocyte: Apoptosis and necrosis | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 3 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 565 | * |
dc.relation.lastpage | 570 | * |
dc.relation.journaltitle | Korean Journal of Physiology and Pharmacology | * |
dc.identifier.scopusid | 2-s2.0-0033368047 | * |
dc.author.google | Sang Hui Chu | * |
dc.author.google | Wol Mi Park | * |
dc.author.google | Kyung Eun Lee | * |
dc.author.google | Young Sook Pae | * |
dc.contributor.scopusid | 이경은(7409769243) | * |
dc.date.modifydate | 20231116115921 | * |