Potential induction of quinone reductase activity of natural products in cultured murine Hepa1c1c7 cells

Abstract

NAD(P)H:quinone reductase (QR), known as DT-diaphorase, is a kind of detoxifying phase II metabolic enzyme catalyzing hydroquinone formation by two electron reduction pathway from quinone type compounds, and thus facilitating excretion of quinoids from human body. With the usefulness of QR induction activity assay system for the modulation of toxicants, in the course of searching for cancer chemopreventive agents from natural products, the methanolic extracts of approximately two hundreds of oriental medicines were primarily evaluated using the induction potential of quinone reductase (QR) activity in cultured murine Hepa1c1c7 cells. As a result, several extracts including Hordeum vulgare, Momordica cochinchinensis, Strychnos ignatii, Houttuynia cordata, and Polygala japonica were found to significantly induce QR activity. In addition, the methylene chloride fraction of H. vulgare, one major dietary food source, showed potent induction of QR activity (CD=6.4 μg/ml). Further study for isolation of active principles from these lead extracts is warranted for the discovery of novel cancer chemopreventive agents. Key words - NAD(P)H:quinone reductase, natural products, murine Hepalclc7 cells, chemopreventive agents.