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Degradation characteristics of methyl ethyl ketone and methyl isobutyl ketone by Pseudomonas putida KT-3

Title
Degradation characteristics of methyl ethyl ketone and methyl isobutyl ketone by Pseudomonas putida KT-3
Authors
Kim M.-J.Lee T.H.Lee K.M.Ryu H.W.Cho K.-S.
Ewha Authors
조경숙
SCOPUS Author ID
조경숙scopus
Issue Date
2002
Journal Title
Korean Journal of Microbiology and Biotechnology
ISSN
1598-642XJCR Link
Citation
Korean Journal of Microbiology and Biotechnology vol. 30, no. 4, pp. 395 - 401
Indexed
SCOPUS; KCI scopus
Document Type
Article
Abstract
Methyl ethyl ketone (MEK) and methyl isobutyl ketone (MIBK) have been widely used as solvents in various industries. Biodegradation of MEK and MIBK by Pseudomonas putida KT-3, which could utilize MEK or MIBK as a sole carbon source, was characterized, and the cosubstrate interaction in MEK/MIBK mixture was also studied. Within the range of initial MEK concentration (from 0.5 to 5. 5 mM), an increased substrate concentration increased the specific degradation rate of MEK by P putida KT-3 (from 3.15 to 10.58 mmol/g DCW · h), but the rate sightly increased at 11.0 mM of initial MEK concentation (11.28 mmol/g DCW · h). The similar degradation rates of MIBK (4.69-4.92 mmol/g DCW · h) were obtained at more than 3.0 mM of initial MIBK concentation. Kinetic analysis on the degradation of MEK/MIBK mixture by P putida KT-3 showed that MEK or MIBK acted as a competitive inhibitor. Maximum degradation rate (Vmax), saturation constant (Km) and inhibition constant (KI) were as follows: Vmax,MEK = 12.94 mmol/g DCW · h; Km,MEK = 1.72 mmol/L; KI,MIBK = 1.30 mmol/L; Vmax,MIBK = 5.00 mmol/g-DCW · h; Km,MIBK = 0.42 mmol/L; KI,MEK = 0.77 mmol/L.
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엘텍공과대학 > 환경공학전공 > Journal papers
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