Evaluation on the P-glycoprotein inhibitory activity of Indonesian medicinal plants

Abstract

One hundred Indonesian plant extracts were screened to investigate their effects on the P-glycoprotein (P-gp) activity in human uterine sarcoma cells, MES-SA/DX5, for the first time. Among others, four samples, Alpinia galanga (BuOH ext.), Sindora sumatrana (CHCl3 ext.), Strychnos ligustrina (CHCl3 ext.), and Zingiber cassumunar Roxb (hexane ext.), exhibited the most potent inhibition on the P-gp activity. They increased cytotoxic activity of daunomycin up to IC50 values of less than 1.41 μM, which is a value with a positive control, verapamil. Other 25 samples showed significant P-gp inhibitory activity with IC50 values between 1.4 and 4.0 μM. These prospective samples will be subjected to further laboratory phytochemical investigation to find active principles.