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Estrogen pretreatment of organotypic hippocampal slices protects neurons against oxygen-glucose deprivation with Akt activation

Title
Estrogen pretreatment of organotypic hippocampal slices protects neurons against oxygen-glucose deprivation with Akt activation
Authors
Park E.-M.Hong S.Lee K.-E.
Ewha Authors
이경은박은미
SCOPUS Author ID
이경은scopus; 박은미scopus
Issue Date
2006
Journal Title
Korean Journal of Physiology and Pharmacology
ISSN
1226-4512JCR Link
Citation
Korean Journal of Physiology and Pharmacology vol. 10, no. 3, pp. 123 - 129
Indexed
SCIE; SCOPUS; KCI scopus
Document Type
Article
Abstract
In several experimental models, estrogens protect neurons against ischemic insults. However, the recent clinical studies of hormone replacement showed negative results to prevent stroke. Therefore, optimal models to study estrogen replacement for neuroprotection are needed before its clinical application. Organotypic hippocampal slice under oxygen-glucose deprivation (OGD) has been established as a model of cerebral ischemia and has advantages to study drug effects. We investigated whether estrogen protected CA1 neurons and affected activation of Akt (pAkt) in CA1 region under OGD. Thus, rat hippocampal slices on day 7 of culture were treated with 17-β estradiol (E, 1 nM) for 7 days before 30 min OGD, and cell death of CA1 neurons was quantified by propidium iodide (PI) staining and expression of pAkt was studied by Western blot and immunofluorescence. PI intensity in slices treated with E was significantly reduced 72 hour after OGD compared to that of non-treated slices (p<0.05). E pretreatment also increased the expression of pAkt 72 hour after OGD compared to that of no treatment (p<0.01). These data suggest that estrogen pretreatment may rescue neurons from ischemic insults through the activation of Akt and also indicate that our model would be a useful alternative method to study the mechanisms and effects of estrogen replacement treatment for neuroprotection.
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의과대학 > 의학과 > Journal papers
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