Analysis of androgen receptor gene by capillary gel eelectrophoresis

Abstract

Capillary gel electrophoresis (CGE) with poly(ethylene oxide) has been applied to the measurement of CAG repeat number in Androgen receptor (AR) gene related to male infertility. Non-linear regression analysis was performed using the standard φXI74 RF/Hae III, 100bp step ladder DNA in order to investigate the exact number of CAG repeat. For 79 Korean infertile males and 89 controls, CAG repeats at exon 1 in AR gene was compared and analyzed by CGE. It turned out that CAG repeat numbers were 24.97±2.6 range, 17-29) for the infertile male, and 23.99±2.4 range, 18-29) for the control, respectively. P value (0.018) was less then 0.05, meaning that the result was statistically meaningful.