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D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity

Title
D-tyrosine negatively regulates melanin synthesis by competitively inhibiting tyrosinase activity
Authors
Park, JisuJung, HyejungKim, KyuriLim, Kyung-MinKim, Ji-youngJho, Eek-hoonOh, Eok-Soo
Ewha Authors
오억수임경민
SCOPUS Author ID
오억수scopus; 임경민scopus
Issue Date
2018
Journal Title
PIGMENT CELL & MELANOMA RESEARCH
ISSN
1755-1471JCR Link1755-148XJCR Link
Citation
vol. 31, no. 3, pp. 374 - 383
Keywords
D-tyrosineL-tyrosinemelaninmelanogenesistyrosinase
Publisher
WILEY
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Although L-tyrosine is well known for its melanogenic effect, the contribution of D-tyrosine to melanin synthesis was previously unexplored. Here, we reveal that, unlike L-tyrosine, D-tyrosine dose-dependently reduced the melanin contents of human MNT-1 melanoma cells and primary human melanocytes. In addition, 500 mu M of D-tyrosine completely inhibited 10 mu M L-tyrosine-induced melanogenesis, and both in vitro assays and L-DOPA staining MNT-1 cells showed that tyrosinase activity is reduced by D-tyrosine treatment. Thus, D-tyrosine appears to inhibit L-tyrosine-mediated melanogenesis by competitively inhibiting tyrosinase activity. Furthermore, we found that D-tyrosine inhibited melanogenesis induced by alpha-MSH treatment or UV irradiation, which are the most common environmental factors responsible for melanin synthesis. Finally, we confirmed that D-tyrosine reduced melanin synthesis in the epidermal basal layer of a 3D human skin model. Taken together, these data suggest that D-tyrosine negatively regulates melanin synthesis by inhibiting tyrosinase activity in melanocyte-derived cells.
DOI
10.1111/pcmr.12668
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자연과학대학 > 생명과학전공 > Journal papers
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