Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서석효 | - |
dc.date.accessioned | 2018-05-30T08:14:28Z | - |
dc.date.available | 2018-05-30T08:14:28Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0363-6135 | - |
dc.identifier.other | OAK-2951 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243656 | - |
dc.description.abstract | The effect of Na+-K+ pump activation on endothelium-dependent relaxation (EDR) and on intracellular Ca2+ concentration ([Ca2+]i) was examined in mouse aorta and mouse aortic endothelial cells (MAECs). The Na+-K+ pump was activated by increasing extracellular K+ concentration ([K +]o) from 6 to 12 mM. In aortic rings, the Na+ ionophore monensin evoked EDR, and this EDR was inhibited by the Na +/Ca2+ exchanger (NCX; reverse mode) inhibitor KB-R7943. Monensin-induced Na+ loading or extracellular Na+ depletion (Na+ replaced by Li+) increased [Ca 2+]i in MAECs, and this increase was inhibited by KB-R7943. Na+-K+ pump activation inhibited EDR and [Ca2+]i increase (K+-induced inhibition of EDR and [Ca2+]i increase). The Na+-K+ pump inhibitor ouabain inhibited K+-induced inhibition of EDR. Monensin (>0.1 μM) and the NCX (forward and reverse mode) inhibitors 2′4′-dichlorobenzamil (> 10 μM) or Ni2+ (>100 μM) inhibited K+-induced inhibition of EDR and [Ca 2+]i increase. KB-R7943 did not inhibit K +-induced inhibition at up to 10 μM but did at 30 μM. In current-clamped MAECs, an increase in [K+]o from 6 to 12 mM depolarized the membrane potential, which was inhibited by ouabain, Ni 2+, or KB-R7943. In aortic rings, the concentration of cGMP was significantly increased by acetylcholine and decreased on increasing [K +]o from 6 to 12 mM. This decrease in cGMP was significantly inhibited by pretreating with ouabain (100 μM), Ni2+ (300 μM), or KB-R7943 (30 μM). These results suggest that activation of the forward mode of NCX after Na+-K+ pump activation inhibits Ca2+ mobilization in endothelial cells, thereby modulating vasomotor tone. Copyright © 2005 the American Physiological Society. | - |
dc.language | English | - |
dc.title | Na+-K+ pump activation inhibits endothelium-dependent relaxation by activating the forward mode of Na+/Ca2+ exchanger in mouse aorta | - |
dc.type | Article | - |
dc.relation.issue | 5 58-5 | - |
dc.relation.volume | 289 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | H2020 | - |
dc.relation.lastpage | H2029 | - |
dc.relation.journaltitle | American Journal of Physiology - Heart and Circulatory Physiology | - |
dc.identifier.doi | 10.1152/ajpheart.00908.2004 | - |
dc.identifier.wosid | WOS:000232497500032 | - |
dc.identifier.scopusid | 2-s2.0-27144490332 | - |
dc.author.google | Kim M.Y. | - |
dc.author.google | Seol G.H. | - |
dc.author.google | Liang G.H. | - |
dc.author.google | Kim J.A. | - |
dc.author.google | Suh S.H. | - |
dc.contributor.scopusid | 서석효(55666113100) | - |
dc.date.modifydate | 20230901081001 | - |