Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 한기환 | * |
dc.date.accessioned | 2018-05-30T08:14:16Z | - |
dc.date.available | 2018-05-30T08:14:16Z | - |
dc.date.issued | 2006 | * |
dc.identifier.issn | 0363-6119 | * |
dc.identifier.other | OAK-3105 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243581 | - |
dc.description.abstract | Urea transport in the kidney is mediated by a family of transporter proteins, including renal urea transporters (UT-A) and erythrocyte urea transporters (UT-B). We aimed to determine whether hydration status affects the subcellular distribution of urea transporters. Male Sprague-Dawley rats were divided into three groups: dehydrated rats (WD) given minimum water, hydrated rats (WL) given 3% sucrose in water for 3 days before death, and control rats given free access to water. We labeled kidney sections with antibodies against UT-A1 and UT-A2 (L194), UT-A3 (Q2), and UT-B using preembedding immunoperoxidase and immunogold methods. In control animals, UT-A1 and UT-A3 immunoreactivities were observed throughout the cytoplasm in inner medullary collecting duct (IMCD) cells, and weak labeling was observed on the basolateral plasma membrane. UT-A2 immunoreactivity in the descending thin limbs (DTL) was observed mainly on the apical and basolateral membranes of type I epithelium, and very faint labeling was observed in the long-loop DTL at the border between the outer and inner medulla. UT-A1 immunoreactivity intensity was markedly lower, and UT-A3 immunoreactivity was higher in IMCD of WD vs. controls. UT-A2 immunoreactivity intensities in the plasma membrane and cytoplasm of type I, II, and III epithelia of DTL were greater in WD vs. controls. In contrast, UT-A1 expression was greater and UT-A 2 and UT-A3 expressions were lower in WL vs. controls. The subcellular distribution of UT-A in DTL or IMCD did not differ between control and experimental animals. UT-B was expressed in the plasma membrane of the descending vasa recta of both control and experimental animals. UT-B intensity was higher in WD and lower in WL vs. controls. These data indicate that changes in hydration status over 3 days affected urea transporter protein expression without changing its subcellular distribution. Copyright © 2006 the American Physiological Society. | * |
dc.language | English | * |
dc.title | Ultrastructural localization of UT-A and UT-B in rat kidneys with different hydration status | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 290 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | R479 | * |
dc.relation.lastpage | R492 | * |
dc.relation.journaltitle | American Journal of Physiology - Regulatory Integrative and Comparative Physiology | * |
dc.identifier.doi | 10.1152/ajpregu.00512.2005 | * |
dc.identifier.wosid | WOS:000234468500027 | * |
dc.identifier.scopusid | 2-s2.0-33644873105 | * |
dc.author.google | Lim S.-W. | * |
dc.author.google | Han K.-H. | * |
dc.author.google | Jung J.-Y. | * |
dc.author.google | Kim W.-Y. | * |
dc.author.google | Yang C.-W. | * |
dc.author.google | Sands J.M. | * |
dc.author.google | Knepper M.A. | * |
dc.author.google | Madsen K.M. | * |
dc.author.google | Kim J. | * |
dc.contributor.scopusid | 한기환(14622504200) | * |
dc.date.modifydate | 20240123095704 | * |