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Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis

Title
Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis
Authors
Seshadri R.M.Klein J.D.Kozlowski S.Sands J.M.Kim Y.-H.Han K.-H.Handlogten M.E.Verlander J.W.Weiner I.D.
Ewha Authors
한기환
SCOPUS Author ID
한기환scopus
Issue Date
2006
Journal Title
American Journal of Physiology - Renal Physiology
ISSN
0363-6127JCR Link
Citation
American Journal of Physiology - Renal Physiology vol. 290, no. 2, pp. F397 - F408
Indexed
SCOPUS WOS scopus
Document Type
Article
Abstract
Chronic metabolic acidosis induces dramatic increases in net acid excretion that are predominantly due to increases in urinary ammonia excretion. The current study examines whether this increase is associated with changes in the expression of the renal ammonia transporter family members, Rh B glycoprotein (Rhbg) and Rh C glycoprotein (Rhcg). Chronic metabolic acidosis was induced in Sprague-Dawley rats by HCl ingestion for 1 wk; control animals were pair-fed. After 1 wk, metabolic acidosis had developed, and urinary ammonia excretion increased significantly. Rhcg protein expression was increased in both the outer medulla and the base of the inner medulla. Intercalated cells in the outer medullary collecting duct (OMCD) and in the inner medullary collecting duct (IMCD) in acid-loaded animals protruded into the tubule lumen and had a sharp, discrete band of apical Rhcg immunoreactivity, compared with a flatter cell profile and a broad band of apical immunolabel in control kidneys. In addition, basolateral Rhcg immunoreactivity was observed in both control and acidotic kidneys. Cortical Rhcg protein expression and immunoreactivity were not detectably altered. Rhcg mRNA expression was not significantly altered in the cortex, outer medulla, or inner medulla by chronic metabolic acidosis. Rhbg protein and mRNA expression were unchanged in the cortex, outer and inner medulla, and no changes in Rhbg immunolabel were evident in these regions. We conclude that chronic metabolic acidosis increases Rhcg protein expression in intercalated cells in the OMCD and in the IMCD, where it is likely to mediate an important role in the increased urinary ammonia excretion.
DOI
10.1152/ajprenal.00162.2005
Appears in Collections:
의과대학 > 의학과 > Journal papers
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