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LIME acts as a transmembrane adapter mediating BCR-dependent B-cell activation
- Title
- LIME acts as a transmembrane adapter mediating BCR-dependent B-cell activation
- Authors
- Ahn E.; Lee H.; Yun Y.
- Ewha Authors
- 윤영대
- SCOPUS Author ID
- 윤영대
- Issue Date
- 2006
- Journal Title
- Blood
- ISSN
- 0006-4971
- Citation
- Blood vol. 107, no. 4, pp. 1521 - 1527
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Assembly of a signaling complex around the transmembrane adapter LAT is essential for the transmission of T-cell receptor (TCR)-mediated signaling. However, a LAT-like molecule responsible for the initial activation events in B-cell receptor (BCR) signaling has not yet been identified. Here, we show that LIME is a transmembrane adaptor required for BCR-mediated B-cell activation. LIME was found to be expressed in mouse splenic B cells. Upon BCR cross-linking, LIME was tyrosine phosphorylated by Lyn and associated with Lyn, Grb2, PLC-γ2, and PI3K. Reduction of LIME expression by the introduction of siRNA resulted in the disruption of BCR-mediated activation of MAPK, calcium flux, NF-AT, PI3K, and NF-κB. Taken together, these results establish that LIME is an essential transmembrane adaptor linking BCR ligation to the downstream signaling events that lead to B-cell activation. © 2006 by The American Society of Hematology.
- DOI
- 10.1182/blood-2005-05-1859
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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