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Sphingosine-1-phosphate-induced intracellular Ca2+ mobilization in human endothelial cells

Title
Sphingosine-1-phosphate-induced intracellular Ca2+ mobilization in human endothelial cells
Authors
Seol G.H.Kim M.Y.Liang G.H.Kim J.A.Kim Y.J.Oh S.Suh S.H.
Ewha Authors
김영주서석효오세관
SCOPUS Author ID
김영주scopus; 서석효scopus; 오세관scopus
Issue Date
2005
Journal Title
Endothelium: Journal of Endothelial Cell Research
ISSN
1062-3329JCR Link
Citation
Endothelium: Journal of Endothelial Cell Research vol. 12, no. 41400, pp. 263 - 269
Indexed
SCOPUS WOS scopus
Document Type
Article
Abstract
The authors have studied the effect of sphingosine-1-phosphate (S1P) on Ca2+ release from intracellular stores in cultured human umbilical vein endothelial cells (HUVECs). In the presence of extracellular Ca2+, S1P increased intracellular Ca2+ concentration ([Ca2+]i) and this increase was partially inhibited by La3+ (1 μM), indicating that S1P induces Ca2+ influx from extracellular pool and Ca2+ release from intracellular stores. S1P increased [Ca2+]i concentration dependently in Ca2+-free extracellular solution. The Hill coefficient (1.7) and EC50 (420 nM) was obtained from the concentration-response relationship. When caffeine depleted Ca2+ store in the presence of ryanodine, S1P did not induce intracellular Ca2+ release. Furthermore, the Ca2+-induced Ca2+ release inhibitors ruthenium red or dantrolene completely inhibited S1P-induced intracellular Ca2+ release. S1P-induced intracellular Ca2+ release was inhibited by the phospholipase C (PLC) inhibitors neomycin and U73312, or the inositol 1,4,5-triphosphate (IP3)-gated Ca2+ channel blocker aminoethoxybiphenyl borane (2-APB). In contrast, S1P-induced intracellular Ca2+ release was not inhibited by the mitochondrial Ca2+ uptake inhibitor CCCP or the mitochondrial Ca2+ release inhibitor cyclosporin A. These results show that S1P mobilizes Ca 2+ from intracellular stores primarily via Ca2+-induced and IP3-induced Ca 2+ release and this Ca2+ mobilization is independent of mitochondrial Ca2+ stores. Copyright © Taylor & Francis Inc.
DOI
10.1080/10623320500476716
Appears in Collections:
의과대학 > 의학과 > Journal papers
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