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Drosophila Immunity: A Large-Scale In Vivo RNAi Screen Identifies Five Serine Proteases Required for Toll Activation

Title
Drosophila Immunity: A Large-Scale In Vivo RNAi Screen Identifies Five Serine Proteases Required for Toll Activation
Authors
Kambris Z.Brun S.Jang I.-H.Nam H.-J.Romeo Y.Takahashi K.Lee W.-J.Ueda R.Lemaitre B.
Ewha Authors
이원재
Issue Date
2006
Journal Title
Current Biology
ISSN
0960-9822JCR Link
Citation
Current Biology vol. 16, no. 8, pp. 808 - 813
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Unlike mammalian Toll-like Receptors, the Drosophila Toll receptor does not interact directly with microbial determinants but is rather activated upon binding a cleaved form of the cytokine-like molecule Spatzle (Spz). During the immune response, Spz is thought to be processed by secreted serine proteases (SPs) present in the hemolymph that are activated by the recognition of gram-positive bacteria or fungi [1]. In the present study, we have used an in vivo RNAi strategy to inactivate 75 distinct Drosophila SP genes. We then screened this collection for SPs regulating the activation of the Toll pathway by gram-positive bacteria. Here, we report the identification of five novel SPs that function in an extracellular pathway linking the recognition proteins GNBP1 and PGRP-SA to Spz. Interestingly, four of these genes are also required for Toll activation by fungi, while one is specifically associated with signaling in response to gram-positive bacterial infections. These results demonstrate the existence of a common cascade of SPs upstream of Spz, integrating signals sent by various secreted recognition molecules via more specialized SPs. © 2006 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.cub.2006.03.020
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일반대학원 > 바이오융합과학과 > Journal papers
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