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JSAP1 is required for the cell adhesion and spreading of mouse embryonic fibroblasts

Title
JSAP1 is required for the cell adhesion and spreading of mouse embryonic fibroblasts
Authors
Chae H.-J.Ha H.-Y.Im J.-Y.Song J.-Y.Park S.Han P.-L.
Ewha Authors
한평림
SCOPUS Author ID
한평림scopus
Issue Date
2006
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
Biochemical and Biophysical Research Communications vol. 345, no. 2, pp. 809 - 816
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
The roles of JSAP1 and JIP1 in cell adhesion and spreading were examined using mouse embryonic fibroblasts (MEFs) deficient in JIP1 (JIP1-KO), JSAP1 (JSAP1-KO), and in both JIP1 and JSAP1 (double-KO), and by using their wild type. After being plated on fibronectin-coated culture plates, wild type MEFs rapidly adhered and differentiated to typical longitudinal fibroblasts in 4 h. JSAP1-KO MEFs showed a similar sequence of adhesion and cell spreading, but their adhesion was weak, and cell spreading sequence proceeded in a delayed manner compared with the wild type. In spreading JSAP1-KO MEFs, adhesion-triggered actin cytoskeleton reorganization and FAK activation proceeded at a slower pace than in wild type MEFs. The cellular properties of double-KO MEFs and JIP1-KO MEFs were similar to those of JSAP1-KO MEFs and wild type MEFs, respectively. These results suggest that JSAP1 plays a role in adhesion and cell spreading by regulating the rapid reorganization of the actin cytoskeleton. © 2006 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bbrc.2006.05.005
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일반대학원 > 뇌·인지과학과 > Journal papers
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