Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오세관 | * |
dc.date.accessioned | 2018-05-18T08:15:25Z | - |
dc.date.available | 2018-05-18T08:15:25Z | - |
dc.date.issued | 2004 | * |
dc.identifier.issn | 0364-3190 | * |
dc.identifier.other | OAK-2493 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243259 | - |
dc.description.abstract | In the present study, we have investigated the effects of prolonged inhibition of nitric oxide synthase (NOS) by infusion of neuronal NOS (nNOS) inhibitor, 7-nitroindazole (7-NI), to examine modulation of NMDA and GABA A receptor binding in rat brain. The duration of sleeping time was significantly increased by the pre-treatment with 7-NI (100 mg/kg) 30 min before pentobarbital (40 mg/kg) treatment in rats. However, the duration of pentobarbital-induced sleep was shortened by the prolonged infusion of 7-NI into cerebroventricle for 7 days. We have investigated the effect of NOS inhibitor on NMDA and GABA A receptor binding characteristics in discrete areas of brain regions by using autoradiographic techniques. The GABA A receptors were analyzed by quantitative autoradiography using [ 3H]muscimol and [ 3H]flunitrazepam binding, and NMDA receptor binding was analyzed by using [ 3H]MK-801 binding in rat brain slices. Rats were infused with 7-NI (500 pmol/10 μl/ h, i.c.v.) for 7 days, through pre-implanted cannula by osmotic minipumps. The levels of [ 3H]muscimol were markedly elevated in cortex, caudate putamen, and thalamus while the levels of [ 3H]flunitrazepam binding were only elevated in cerebellum by NOS inhibitor. However, there was no change in the level of [ 3H]MK-801 binding except decreasing in the thalamus. These results show that the prolonged inhibition of NOS by 7-NI-infusion highly elevates [ 3H]muscimol binding in a region-specific manner and decreases the pentobarbital-induced sleep. | * |
dc.language | English | * |
dc.subject | autoradiography | * |
dc.subject | flunitrazepam | * |
dc.subject | GABA A receptor | * |
dc.subject | muscimol | * |
dc.subject | nNOS | * |
dc.subject | osmotic pump | * |
dc.title | Changes of [ 3H]muscimol, [ 3H]flunitrazepam and [ 3H]MK-801 binding in rat brain by prolonged ventricular infusion of 7-nitroindazole | * |
dc.type | Article | * |
dc.relation.issue | 12 | * |
dc.relation.volume | 29 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2221 | * |
dc.relation.lastpage | 2229 | * |
dc.relation.journaltitle | Neurochemical Research | * |
dc.identifier.doi | 10.1007/s11064-004-7029-z | * |
dc.identifier.wosid | WOS:000225573500005 | * |
dc.identifier.scopusid | 2-s2.0-11144339424 | * |
dc.author.google | Kim E.Y. | * |
dc.author.google | Shin K.M. | * |
dc.author.google | Jang S. | * |
dc.author.google | Oh S. | * |
dc.contributor.scopusid | 오세관(7404103757) | * |
dc.date.modifydate | 20240118133340 | * |