View : 173 Download: 9

The major target of the endogenously generated reactive oxygen species in response to insulin stimulation is Phosphatase and Tensin homolog and not phosphoinositide-3 kinase (PI-3 kinase) in the PI-3 kinase/Akt pathway

Title
The major target of the endogenously generated reactive oxygen species in response to insulin stimulation is Phosphatase and Tensin homolog and not phosphoinositide-3 kinase (PI-3 kinase) in the PI-3 kinase/Akt pathway
Authors
Seo J.H.Ahn Y.Lee S.-R.Yeo C.Y.Hur K.C.
Ewha Authors
허규정여창열
SCOPUS Author ID
허규정scopus; 여창열scopus
Issue Date
2005
Journal Title
Molecular Biology of the Cell
ISSN
1059-1524JCR Link
Citation
Molecular Biology of the Cell vol. 16, no. 1, pp. 348 - 357
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Phosphoinositide-3 kinase (PI-3 kinase) and its downstream signaling molecules PDK-1 and Akt were analyzed in SK-N-SH and SK-N-BE(2) human neuroblastoma cell lines. When cells were stimulated with insulin, PI-3 kinase was activated in both cell lines, whereas the translocation of PDK-1 to the membrane fraction and phosphorylated Akt were observed only in SK-N-SH cells. Analyses of the insulin-mediated reactive oxygen species (ROS) generation and Phosphatase and Tensin homolog (PTEN) oxidation indicate that PTEN oxidation occurred in SK-N-SH cells, which can produce ROS, but not in SK-N-BE(2) cells, which cannot increase ROS in response to insulin stimulation. When SK-N-SH cells were pretreated with the NADPH oxidase inhibitor diphenyleneiodonium chloride before insulin stimulation, insulin-mediated translocation of PDK-1 to the membrane fraction and phosphorylation of Akt were remarkably reduced, whereas PI-3 kinase activity was not changed significantly. These results indicate that not only PI-3 kinase activation but also inhibition of PTEN by ROS is needed to increase cellular level of phosphatidylinositol 3,4,5-trisphosphate for recruiting downstream signaling molecules such as PDK-1 and Akt in insulin-mediated signaling. Moreover, the ROS generated by insulin stimulation mainly contributes to the inactivation of PTEN and not to the activation of PI-3 kinase in the PI-3 kinase/Akt pathway.
DOI
10.1091/mbc.E04-05-0369
Appears in Collections:
일반대학원 > 바이오융합과학과 > Journal papers
Files in This Item:
The major target.pdf(419.53 kB) Download
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE