Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 강덕희 | - |
dc.date.accessioned | 2018-05-18T08:15:12Z | - |
dc.date.available | 2018-05-18T08:15:12Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1046-6673 | - |
dc.identifier.other | OAK-2643 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243179 | - |
dc.description.abstract | Thrombospondin-1 (TSP-1) inhibits angiogenesis and activates latent TGF-β1, both of which are strongly associated with progression of renal disease. Recently, it was reported that Smad2 but not Smad3 regulates TSP-1 expression in response to TGF-β1 in rat tubular epithelial cells as well as in mouse fibroblasts. This study investigated the role of ERK1/2 and p38 mitogen-activated protein kinases (MAPK). TGF-β1 activated both ERK1/2 and p38 in the rat proximal tubular cell line NRK52E. Blocking ERK1/2 and p38 inhibited TGF-β1-induced TSP-1 mRNA and protein expression. Next, the cross-talk between Smad2 and ERK1/2 or p38 was examined. Whereas blocking of ERK1/2 or p38 failed to inhibit TGF-β1-induced Smad2 activation, inhibition of Smad2 by Smad7 overexpression inhibited the phosphorylation of ERK1/2 but not p38 in response to TGF-β1. Similar results were observed using mouse fibroblasts from Smad2 knockout embryos, in that TGF-β1 was able to activate p38 but not ERK1/2 in this cell line. In conclusion, TSP-1 expression is regulated by both ERK1/2 and p38 MAPK in rat proximal tubular cells and mouse fibroblasts in response to TGF-β1. The ERK1/2 activation is dependent on Smad2 activation, whereas the p38 activation occurs independent of Smad2. Because TSP-1 is a major antiangiogenic molecule and an activator of TGF-β1, this provides an important insight to the mechanism by which TGF-β1 may mediate interstitial fibrosis and progressive renal disease. Copyright © 2005 by the American Society of Nephrology. | - |
dc.language | English | - |
dc.title | Role of ERK1/2 and p38 mitogen-activated protein kinases in the regulation of thrombospondin-1 by TGF-β1 in rat proximal tubular cells and mouse fibroblasts | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 16 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 899 | - |
dc.relation.lastpage | 904 | - |
dc.relation.journaltitle | Journal of the American Society of Nephrology | - |
dc.identifier.doi | 10.1681/ASN.2004080689 | - |
dc.identifier.wosid | WOS:000227935800012 | - |
dc.identifier.scopusid | 2-s2.0-24344491188 | - |
dc.author.google | Nakagawa T. | - |
dc.author.google | Lan H.Y. | - |
dc.author.google | Glushakova O. | - |
dc.author.google | Zhu H.J. | - |
dc.author.google | Kang D.-H. | - |
dc.author.google | Schreiner G.F. | - |
dc.author.google | Bottinger E.P. | - |
dc.author.google | Johnson R.J. | - |
dc.author.google | Sautin Y.Y. | - |
dc.contributor.scopusid | 강덕희(17233695600) | - |
dc.date.modifydate | 20230210140157 | - |