Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 정성철 | * |
dc.date.accessioned | 2018-05-18T08:14:57Z | - |
dc.date.available | 2018-05-18T08:14:57Z | - |
dc.date.issued | 2005 | * |
dc.identifier.issn | 1099-498X | * |
dc.identifier.other | OAK-2835 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/243082 | - |
dc.description.abstract | Background: Gaucher disease is a lysosomal storage disorder resulting from a deficiency of glucocerebrosidase (GC). Recently, lentivirus vectors have been developed for efficient gene transfer into hematopoietic stem cells (HSCs). A recombinant lentivirus vector was used to evaluate the transduction of the human GC gene into murine bone-marrow-derived HSCs and its expression in their progeny. Methods: Murine HSCs were transduced with lentivirus vector (lenti-EF-GC; MOI = 10-100). We transplanted female wild-type C57BL/6J mice with genetically modified male HSCs via the tail vein. Results: We show that intravenous transplantation of transduced HSCs has therapeutic potential. Enzyme activity was increased two- to three-fold in various tissues, especially in the hematopoietic system. Numerous transplanted HSCs survived for 6 months and were shown by PCR to contain the provirus genes; the Y chromosome was identified by FISH analysis in the cells of female mouse recipients. Conclusions: The recombinant lentiviral vector transduces HSCs that are capable of long-term gene expression in vivo. This approach is potentially useful for the treatment of patents with Gaucher disease and other lysosomal storage disorders. Copyright © 2005 John Wiley & Sons, Ltd. | * |
dc.language | English | * |
dc.title | Long-term expression of the human glucocerebrosidase gene in vivo after transplantation of bone-marrow-derived cells transformed with a lentivirus vector | * |
dc.type | Article | * |
dc.relation.issue | 7 | * |
dc.relation.volume | 7 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 878 | * |
dc.relation.lastpage | 887 | * |
dc.relation.journaltitle | Journal of Gene Medicine | * |
dc.identifier.doi | 10.1002/jgm.732 | * |
dc.identifier.wosid | WOS:000230695800005 | * |
dc.identifier.scopusid | 2-s2.0-22544444969 | * |
dc.author.google | Kim E.Y. | * |
dc.author.google | Hong Y.B. | * |
dc.author.google | Lai Z. | * |
dc.author.google | Cho Y.-H. | * |
dc.author.google | Brady R.O. | * |
dc.author.google | Jung S.-C. | * |
dc.contributor.scopusid | 정성철(57008539100) | * |
dc.date.modifydate | 20240422114306 | * |