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dc.contributor.author배윤수-
dc.date.accessioned2018-05-18T08:14:54Z-
dc.date.available2018-05-18T08:14:54Z-
dc.date.issued2005-
dc.identifier.issn0898-6568-
dc.identifier.otherOAK-2869-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/243060-
dc.description.abstractPhospholipase C-γ1 (PLC-γ1) plays pivotal roles in cellular growth and proliferation. Upon the stimulation of growth factors and hormones, PLC-γ1 is rapidly phosphorylated at three known sites; Tyr771, Tyr783 and Tyr1254 and its enzymatic activity is up-regulated. In this study, we demonstrate for the first time that Grb2, an adaptor protein, specifically interacts with tyrosine-phosphorylated PLC-γ1 at Tyr783. The association of Grb2 with PLC-γ1 was induced by the treatment with epidermal growth factor (EGF). Replacement of Tyr783 with Phe completely blocked EGF-induced interaction of PLC-γ1 with Grb2, indicating that tyrosine phosphorylation of PLC-γ1 at Tyr783 is essential for the interaction with Grb2. Interestingly, the depletion of Grb2 from HEK-293 cells by RNA interference significantly enhanced increased EGF-induced PLC-γ1 enzymatic activity and mobilization of the intracellular Ca2+, while it did not affect EGF-induced tyrosine phosphorylation of PLC-γ1. Furthermore, overexpression of Grb2 inhibited PLC-γ1 enzymatic activity. Taken together, these results suggest Grb2, in addition to its key function in signaling through Ras, may have a negatively regulatory role on EGF-induced PLC-γ1 activation. © 2005 Elsevier Inc. All rights reserved.-
dc.languageEnglish-
dc.titleGrb2 negatively regulates epidermal growth factor-induced phospholipase C-γ1 activity through the direct interaction with tyrosine-phosphorylated phospholipase C-γ1-
dc.typeArticle-
dc.relation.issue10-
dc.relation.volume17-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage1289-
dc.relation.lastpage1299-
dc.relation.journaltitleCellular Signalling-
dc.identifier.doi10.1016/j.cellsig.2005.01.005-
dc.identifier.wosidWOS:000231182900012-
dc.identifier.scopusid2-s2.0-22144486622-
dc.author.googleJang H.C.-
dc.author.googleHong W.-P.-
dc.author.googleYun S.-
dc.author.googleHyeon S.K.-
dc.author.googleLee J.-R.-
dc.author.googleJong B.P.-
dc.author.googleYun S.B.-
dc.author.googleSung H.R.-
dc.author.googleSuh P.-G.-
dc.contributor.scopusid배윤수(15031067200)-
dc.date.modifydate20190901081003-
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자연과학대학 > 생명과학전공 > Journal papers
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