Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | - |
dc.date.accessioned | 2018-05-02T08:15:48Z | - |
dc.date.available | 2018-05-02T08:15:48Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.other | OAK-2095 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/242811 | - |
dc.description.abstract | During cell-matrix adhesion, syndecan-4 transmembrane heparan sulphate proteoglycan plays a critical role in the formation of focal adhesions and stress fibres. We have shown previously that the syndecan-4 cytoplasmic domain directly binds to and activates PKC-α (protein kinase C-α) in vitro [Oh, Woods and Couchman (1997) J. Biol. Chem. 272, 8133-8136]. However, whether syndecan-4 has the same activity in vivo needs to be addressed. Using mammalian two-hybrid assays, we showed that syndecan-4 interacted with PKC-α in vivo and that this interaction was mediated through syndecan-4 cytoplasmic domain. Furthermore, the activation of PKC increased the extent of interaction between syndecan-4 and PKC-α. Overexpression of syndecan-4, but not a mutant lacking its cytoplasmic domain, specifically increased the level of endogenous PKC-α and enhanced the translocation of PKC-α into both detergent-insoluble and membrane fractions. In addition, rat embryo fibroblasts overexpressing syndecan-4 exhibited a slowed down-regulation of PKC-α in response either to a prolonged treatment with PMA or to maintaining cells in suspension culture. PKC-α immunocomplex kinase assays also showed that syndecan-4 overexpression increased the activity of membrane PKC-α. Taken together, these results suggest that syndecan-4 interacts with PKC-α in vivo and regulates its locali-zation, activity and stability. | - |
dc.language | English | - |
dc.title | Syndecan-4 regulates localization, activity and stability of protein kinase C-α | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 378 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1007 | - |
dc.relation.lastpage | 1014 | - |
dc.relation.journaltitle | Biochemical Journal | - |
dc.identifier.doi | 10.1042/BJ20031734 | - |
dc.identifier.wosid | WOS:000220481100035 | - |
dc.identifier.scopusid | 2-s2.0-1642545636 | - |
dc.author.google | Keum E. | - |
dc.author.google | Kim Y. | - |
dc.author.google | Kim J. | - |
dc.author.google | Kwon S. | - |
dc.author.google | Lim Y. | - |
dc.author.google | Han I. | - |
dc.author.google | Oh E.-S. | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.date.modifydate | 20190901081003 | - |