Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이서구 | * |
dc.contributor.author | 배윤수 | * |
dc.contributor.author | 이원재 | * |
dc.date.accessioned | 2018-05-02T08:15:40Z | - |
dc.date.available | 2018-05-02T08:15:40Z | - |
dc.date.issued | 2004 | * |
dc.identifier.issn | 0270-7306 | * |
dc.identifier.other | OAK-2169 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/242768 | - |
dc.description.abstract | The generation of reactive oxygen species (ROS) in cells stimulated with growth factors requires the activation of phosphatidylinositol 3-kinase (PI3K) and the Rac protein. We report here that the COOH-terminal region of Nox1, a protein related to gp91phox (Nox2) of phagocytic cells, is constitutively associated with βPix, a guanine nucleotide exchange factor for Rac. Both growth factor-induced ROS production and Rac1 activation were completely blocked in cells depleted of βPix by RNA interference. Rac1 was also shown to bind to the COOH-terminal region of Nox1 in a growth factor-dependent manner. Moreover, the depletion of Nox1 by RNA interference inhibited growth factor-induced ROS generation. These results suggest that ROS production in growth factor-stimulated cells is mediated by the sequential activation of PI3K, βPix, and Rac1, which then binds to Nox1 to stimulate its NADPH oxidase activity. Copyright © 2004, American Society for Microbiology. All Rights Reserved. | * |
dc.language | English | * |
dc.title | Sequential activation of phosphatidylinositol 3-kinase, βPix, Rac1, and Nox1 in growth factor-induced production of H2O2 | * |
dc.type | Article | * |
dc.relation.issue | 10 | * |
dc.relation.volume | 24 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 4384 | * |
dc.relation.lastpage | 4394 | * |
dc.relation.journaltitle | Molecular and Cellular Biology | * |
dc.identifier.doi | 10.1128/MCB.24.10.4384-4394.2004 | * |
dc.identifier.wosid | WOS:000221440900027 | * |
dc.identifier.scopusid | 2-s2.0-2942628119 | * |
dc.author.google | Hye S.P. | * |
dc.author.google | Seung H.L. | * |
dc.author.google | Park D. | * |
dc.author.google | Jun S.L. | * |
dc.author.google | Sung H.R. | * |
dc.author.google | Won J.L. | * |
dc.author.google | Sue G.R. | * |
dc.author.google | Yun S.B. | * |
dc.contributor.scopusid | 이서구(7401852092) | * |
dc.contributor.scopusid | 배윤수(15031067200) | * |
dc.contributor.scopusid | 이원재(57171092600) | * |
dc.date.modifydate | 20240423081003 | * |