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Sequential activation of phosphatidylinositol 3-kinase, βPix, Rac1, and Nox1 in growth factor-induced production of H2O2

Title
Sequential activation of phosphatidylinositol 3-kinase, βPix, Rac1, and Nox1 in growth factor-induced production of H2O2
Authors
Hye S.P.Seung H.L.Park D.Jun S.L.Sung H.R.Won J.L.Sue G.R.Yun S.B.
Ewha Authors
이서구배윤수이원재
SCOPUS Author ID
이서구scopusscopus; 배윤수scopus
Issue Date
2004
Journal Title
Molecular and Cellular Biology
ISSN
0270-7306JCR Link
Citation
Molecular and Cellular Biology vol. 24, no. 10, pp. 4384 - 4394
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
The generation of reactive oxygen species (ROS) in cells stimulated with growth factors requires the activation of phosphatidylinositol 3-kinase (PI3K) and the Rac protein. We report here that the COOH-terminal region of Nox1, a protein related to gp91phox (Nox2) of phagocytic cells, is constitutively associated with βPix, a guanine nucleotide exchange factor for Rac. Both growth factor-induced ROS production and Rac1 activation were completely blocked in cells depleted of βPix by RNA interference. Rac1 was also shown to bind to the COOH-terminal region of Nox1 in a growth factor-dependent manner. Moreover, the depletion of Nox1 by RNA interference inhibited growth factor-induced ROS generation. These results suggest that ROS production in growth factor-stimulated cells is mediated by the sequential activation of PI3K, βPix, and Rac1, which then binds to Nox1 to stimulate its NADPH oxidase activity. Copyright © 2004, American Society for Microbiology. All Rights Reserved.
DOI
10.1128/MCB.24.10.4384-4394.2004
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일반대학원 > 생명·약학부 > Journal papers
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