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Purification and structural determination of hematopoietic stem cell-stimulating monoacetyldiglycerides from Cervus nippon (deer antler)

Title
Purification and structural determination of hematopoietic stem cell-stimulating monoacetyldiglycerides from Cervus nippon (deer antler)
Authors
Yang H.O.Kim S.H.Cho S.-H.Kim M.-G.Seo J.-Y.Park J.-S.Jhon G.-J.Han S.-Y.
Ewha Authors
전길자한소엽
SCOPUS Author ID
전길자scopus; 한소엽scopus
Issue Date
2004
Journal Title
Chemical and Pharmaceutical Bulletin
ISSN
0009-2363JCR Link
Citation
Chemical and Pharmaceutical Bulletin vol. 52, no. 7, pp. 874 - 878
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
A mixture of monoacetyldiglycerides was newly isolated from the chloroform extract of antlers of Cervus nippon, guided by the hematopoietic stimulation of stem cells. The structures of monoacetyldiglycerides were determined by various spectroscopic methods: FAB MS, CID tandem MS, and 1D and 2D NMR. A mixture of at least nine inseparable sn-3-monoacetyldiglycerides was identified: 1 [C 39H 72O 6 (C16 : 0/C18 : 1)], 2 [C 39H 72O 6 (C18 : 1/C16 : 0)], 3 [C 39H 70O 6 (C16 : 0/C18 : 2)], 4 [C 39H 70O 6 (C18 : 2/C16 : 0)], 5 [C 41H 74O 6 (C18 : 0/C18 : 2), 6 [C 41H 74O 6 (C18 : 2/C18 : 0)], 7 [C 41H 74O 6 (C18 : 1/C18 : 1)], 8 [C 43H 74O 6 (C18 : 0/C20 : 4)], and 9 [C 43H 74O 6 (C20 : 4/C18 : 0)]. Among these nine monoacetyldiglycerides in deer antlers, compound 3 was one of the major compounds and was efficiently synthesized from glycerol. Spectral data of synthetic monoacetyldiglyceride 3 were compared with the corresponding data for the mixture of natural monoacetyldiglycerides. The mixture of natural monoacetyldiglycerides from deer antlers showed potent activity on the hematopoiesis (stimulation index=1.40±0.05, p<0.02 at 1 μg/ml), and synthetic monoacetyldiglyceride 3 showed even better activity (stimulation index=1.54±0.12, p<0.001, at 1 μg/ml). © 2004 Pharmaceutical Society of Japan.
DOI
10.1248/cpb.52.874
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자연과학대학 > 화학·나노과학전공 > Journal papers
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