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The RING-H2-finger protein APC11 as a target of hydrogen peroxide

Title
The RING-H2-finger protein APC11 as a target of hydrogen peroxide
Authors
Chang T.-S.Jeong W.Lee D.-Y.Cho C.-S.Rhee S.G.
Ewha Authors
이서구정우진창동신
SCOPUS Author ID
이서구scopusscopus; 정우진scopus; 창동신scopus
Issue Date
2004
Journal Title
Free Radical Biology and Medicine
ISSN
0891-5849JCR Link
Citation
Free Radical Biology and Medicine vol. 37, no. 4, pp. 521 - 530
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
The anaphase-promoting complex (APC) is a ubiquitin-protein ligase (E3) that targets cell cycle regulators such as cyclin B and securin for degradation. The APC11 subunit functions as the catalytic core of this complex and mediates the transfer of ubiquitin from a ubiquitin-conjugating enzyme (E2) to the substrate. APC11 contains a RING-H2-finger domain, which includes one histidine and seven cysteine residues that coordinate two Zn2+ ions. We now show that exposure of purified APC11 to H2O2 (0.1 to 1 mM) induced the release of bound zinc as a result of the oxidation of cysteine residues. It also impaired the physical interaction between APC11 and the E2 enzyme Ubc4 as well as inhibited the ubiquitination of cyclin B1 by APC11. The release of HeLa cells from metaphase arrest in the presence of exogenous H 2O2 inhibited the ubiquitination of cyclin B1 as well as the degradation of cyclin B1 and securin that were apparent in the absence of H2O2. The presence of H2O2 also blocked the co-immunoprecipitation of Ubc4 with APC11 and delayed the exit of cells from mitosis. Inhibition of APC11 function by H2O2 thus likely contributes to the delay in cell cycle progression through mitosis that is characteristic of cells subjected to oxidative stress.
DOI
10.1016/j.freeradbiomed.2004.05.006
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일반대학원 > 생명·약학부 > Journal papers
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