Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 한평림 | - |
dc.date.accessioned | 2018-05-02T08:15:28Z | - |
dc.date.available | 2018-05-02T08:15:28Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0026-895X | - |
dc.identifier.other | OAK-2315 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/242708 | - |
dc.description.abstract | The pretreatment of cultured cortical neurons with neurotrophic factors markedly potentiates the cytotoxicity induced by low concentrations of Zn 2+ or excitotoxins. In the current study, we investigated the mechanism underlying the insulin-like growth factor-I (IGF-I)-induced Zn 2+ toxicity potentiation. The pretreatment of primary cortical cultures for more than 12 h with 100 ng/ml of IGF-I increased the cytotoxicity induced by 80 μM Zn2+ by more than 2-fold. The IGF-I-enhanced cell death was blocked by the COX-2-specific inhibitors N-[2-(cyclohexyloxyl)-4- nitrophenyl]-methane sulfonamide (NS-398; 10-100 μM) and 1-[(4- methylsulfonyl)phenyl]-3-trifluoro-methyl-5-[(4-fluoro)phenyl]pyrazole (SC58125; 10 μM) and by the antioxidant trolox (30 μM). In addition, it was observed that COX-2 expression was increased 12 to 24 h after IGF-I treatment. Preincubation of cortical cultures with IGF-I increased arachidonic acid (AA)-induced cytotoxicity, and AA increased Zn2+ toxicity, which suggested the involvement of COX activity in these cellular responses. Moreover, enhanced COX-2 activity led to a decrease in the cell's reducing power, as indicated by a gradual depletion of intracellular GSH. Cortical neurons pretreated with IGF-I and then Zn2+ showed consistently enhanced reactive oxygen species production, which was repressed by NS-398 and SC58125. Cortical neurons treated with Zn2+ and then AA displayed the increased ROS production, which was also suppressed by NS-398 and SC58125. These results suggest that COX-2 is an endogenous factor responsible for the IGF-I-induced potentiation of Zn2+ toxicity and that enhanced COX-2 activity leads to a decrease in the cell's reducing power and an increase in ROS accumulation in primary cortical cultures. | - |
dc.language | English | - |
dc.title | COX-2 regulates the insulin-like growth factor I-induced potentiation of Zn2+-toxicity in primary cortical culture | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 66 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 368 | - |
dc.relation.lastpage | 376 | - |
dc.relation.journaltitle | Molecular Pharmacology | - |
dc.identifier.doi | 10.1124/mol.66.3. | - |
dc.identifier.wosid | WOS:000223443500002 | - |
dc.identifier.scopusid | 2-s2.0-4944244055 | - |
dc.author.google | Im J.-Y. | - |
dc.author.google | Kim D. | - |
dc.author.google | Lee K.-W. | - |
dc.author.google | Kim J.-B. | - |
dc.author.google | Lee J.-K. | - |
dc.author.google | Dong S.K. | - |
dc.author.google | Young I.L. | - |
dc.author.google | Ha K.-S. | - |
dc.author.google | Joe C.O. | - |
dc.author.google | Han P.-L. | - |
dc.contributor.scopusid | 한평림(7201947605) | - |
dc.date.modifydate | 20230901081001 | - |