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Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation

Title
Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation
Authors
Kim, Min SooLee, SangminYun, SangukSuh, Pann-GhillPark, JongmiCui, MinghuaChoi, SunCha, Sun-ShinJin, Wook
Ewha Authors
최선차선신
SCOPUS Author ID
최선scopus; 차선신scopus
Issue Date
2018
Journal Title
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN
0141-8130JCR Link1879-0003JCR Link
Citation
vol. 107, pp. 1650 - 1658
Keywords
TartrateDJ-1Parkinson's diseaseInorganic phosphateROS
Publisher
ELSEVIER SCIENCE BV
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The DJ-1 protein engages in diverse cellular and pathological processes, including tumorigenesis, apoptosis, sperm fertilization, and the progression of Parkinson's disease (PD). The functional dimeric form of DJ-1 transforms into non-functional filamentous aggregates in an inorganic phosphate (P-1)-dependent manner in vitro. Here, we demonstrated that P-i and reactive oxygen species (ROS) induce DJ-1 aggregation in Neuro2A and SH-SY5Y cells. Remarkably, tartrate treatment significantly reduced P-i- and ROS-induced DJ-1 aggregation and restored P-i- and ROS-provoked cell death using quantitative data as mean +/- standard deviation, and statistics. Mechanistically, tartrate prevented DJ-1 aggregation via occupying the P-i-binding site. These findings revealed an unexpected physiological role of tartrate in the maintenance of DJ-1 function, and thus, a potential use as an inhibitor of DJ-1 aggregation. (C) 2017 Elsevier B.V. All rights reserved.
DOI
10.1016/j.ijbiomac.2017.10.022
Appears in Collections:
약학대학 > 약학과 > Journal papers
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