Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 차선신 | * |
dc.date.accessioned | 2018-04-25T08:13:39Z | - |
dc.date.available | 2018-04-25T08:13:39Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 0006-291X | * |
dc.identifier.issn | 1090-2104 | * |
dc.identifier.other | OAK-21877 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/242572 | - |
dc.description.abstract | DJ-1 is a multifunctional protein associated with Parkinson's disease (PD) and tumorigenesis. In response to ultraviolet B (UVB) irradiation, DJ-1 is translocated into the mitochondria, and its interaction with the mitochondrial protein Bcl-X-L protects cells against death. In this study, we characterized the molecular interaction between DJ-1 and Bcl-X-L by NMR spectroscopy. The NMR chemical shift perturbation data demonstrated that the oxidized but not the reduced form of DJ-1 binds to the predominantly hydrophobic groove surrounded by the BH1-BH3 domains in Bcl-X-L. In addition, our results showed that the C-terminal alpha 8-helix peptide (Cpep) of DJ-1 binds to the pro-apoptotic BH3 peptide-binding hydrophobic groove in Bcl-X-L and, thus, acts as a Bcl-X-L-binding motif. In combination with the NMR chemical shift perturbation data, a refined structural model of the Bcl-X-L/DJ-1 Cpep complex revealed that the binding mode is remarkably similar to that of other Bcl-X-L/pro-apoptotic BH3 peptide complexes. Taken together, our results provide a structural basis for the binding mechanism between DJ-1 and Bcl-X-L, which will contribute to molecular understanding of the role of mitochondrial DJ-1 in Bcl-X-L regulation in response to oxidative stress. (C) 2017 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | * |
dc.subject | Bcl-X-L | * |
dc.subject | DJ-1 | * |
dc.subject | NMR spectroscopy | * |
dc.subject | Protein interaction | * |
dc.subject | Complex structure | * |
dc.title | Structural basis for the interaction between DJ-1 and Bcl-X-L | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 495 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1067 | * |
dc.relation.lastpage | 1073 | * |
dc.relation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | * |
dc.identifier.doi | 10.1016/j.bbrc.2017.11.129 | * |
dc.identifier.wosid | WOS:000423897600161 | * |
dc.identifier.scopusid | 2-s2.0-85035087795 | * |
dc.author.google | Lee, Mi-Kyung | * |
dc.author.google | Lee, Min-Sung | * |
dc.author.google | Bae, Da-Woon | * |
dc.author.google | Lee, Dong-Hwa | * |
dc.author.google | Cha, Sun-Shin | * |
dc.author.google | Chi, Seung-Wook | * |
dc.contributor.scopusid | 차선신(7201864593) | * |
dc.date.modifydate | 20240222163943 | * |