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The developmental transcriptome atlas of the spoon worm Urechis unicinctus (Echiurida: Annelida)

Title
The developmental transcriptome atlas of the spoon worm Urechis unicinctus (Echiurida: Annelida)
Authors
Park, ChungooHan, Yong-HeeLee, Sung-GwonRy, Kyoung-BinOh, JooseongKern, Elizabeth M. A.Park, Joong-KiCho, Sung-Jin
Ewha Authors
박중기
SCOPUS Author ID
박중기scopus
Issue Date
2018
Journal Title
GIGASCIENCE
ISSN
2047-217XJCR Link
Citation
GIGASCIENCE vol. 7, no. 3
Keywords
Urechis unicinctusEchiuridadevelopmental transcriptomeRNA-Seqde novo assembly
Publisher
OXFORD UNIV PRESS
Indexed
SCIE; SCOPUS WOS
Document Type
Article

Data Paper
Abstract
Background: Echiurida is one of the most intriguing major subgroups of annelida because, unlike most other annelids, echiurids lack metameric body segmentation as adults. For this reason, transcriptome analyses from various developmental stages of echiurid species can be of substantial value for understanding precise expression levels and the complex regulatory networks during early and larval development. Results: A total of 914 million raw RNA-Seq reads were produced from 14 developmental stages of Urechis unicinctus and were de novo assembled into contigs spanning 63,928,225 bp with an N50 length of 2700 bp. The resulting comprehensive transcriptome database of the early developmental stages of U. unicinctus consists of 20,305 representative functional protein-coding transcripts. Approximately 66% of unigenes were assigned to superphylum-level taxa, including Lophotrochozoa (40%). The completeness of the transcriptome assembly was assessed using benchmarking universal single-copy orthologs; 75.7% of the single-copy orthologs were presented in our transcriptome database. We observed 3 distinct patterns of global transcriptome profiles from 14 developmental stages and identified 12,705 genes that showed dynamic regulation patterns during the differentiation and maturation of U. unicinctus cells. Conclusions: We present the first large-scale developmental transcriptome dataset of U. unicinctus and provide a general overview of the dynamics of global gene expression changes during its early developmental stages. The analysis of time-course gene expression data is a first step toward understanding the complex developmental gene regulatory networks in U. unicinctus and will furnish a valuable resource for analyzing the functions of gene repertoires in various developmental phases.
DOI
10.1093/gigascience/giy007
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일반대학원 > 에코과학부 > Journal papers
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