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Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine

Title
Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine
Authors
Kwon, HyokyoungKim, MinjeongSeo, YunmiMoon, Yae SeulLee, Hwa JeongLee, KyuriLee, Hyukjin
Ewha Authors
이화정이혁진이규리
SCOPUS Author ID
이화정scopus; 이혁진scopus
Issue Date
2018
Journal Title
BIOMATERIALS
ISSN
0142-9612JCR Link1878-5905JCR Link
Citation
vol. 156, pp. 172 - 193
Keywords
Synthetic mRNAIn vitro transcription (IVT)Cellular reprogramingRegenerative medicine
Publisher
ELSEVIER SCI LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The field of gene therapy has evolved over the past two decades after the first introduction of nucleic acid drugs, such as plasmid DNA (pDNA). With the development of in vitro transcription (IVT) methods, synthetic mRNA has become an emerging class of gene therapy. IVT mRNA has several advantages over conventional pDNA for the expression of target proteins. mRNA does not require nuclear localization to mediate protein translation. The intracellular process for protein expression is much simpler and there is no potential risk of insertion mutagenesis. Having these advantages, the level of protein expression is far enhanced as comparable to that of viral expression systems. This makes IVT mRNA a powerful alternative gene expression system for various applications in regenerative medicine. In this review, we highlight the synthesis and preparation of IVT mRNA and its therapeutic applications. The article includes the design and preparation of IVT mRNA, chemical modification of IVT mRNA, and therapeutic applications of IVT mRNA in cellular reprogramming, stem cell engineering, and protein replacement therapy. Finally, future perspectives and challenges of IVT mRNA are discussed. (C) 2017 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.biomaterials.2017.11.034
Appears in Collections:
약학대학 > 약학과 > Journal papers
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