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Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation
- Inhibitory effect of tartrate against phosphate-induced DJ-1 aggregation
- Choi, Sun; Cha, Sun-Shin; Jin, Wook; Kim, Min Soo; Lee, Sangmin; Yun, Sanguk; Suh, Pann-Ghill; Park, Jongmi; Cui, Minghua
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- 0141-8130; 1879-0003
- vol. 107, pp. 1650 - 1658
- Tartrate; DJ-1; Parkinson's disease; Inorganic phosphate; ROS
- ELSEVIER SCIENCE BV
- SCI; SCIE; SCOPUS
- The DJ-1 protein engages in diverse cellular and pathological processes, including tumorigenesis, apoptosis, sperm fertilization, and the progression of Parkinson's disease (PD). The functional dimeric form of DJ-1 transforms into non-functional filamentous aggregates in an inorganic phosphate (P-1)-dependent manner in vitro. Here, we demonstrated that P-i and reactive oxygen species (ROS) induce DJ-1 aggregation in Neuro2A and SH-SY5Y cells. Remarkably, tartrate treatment significantly reduced P-i- and ROS-induced DJ-1 aggregation and restored P-i- and ROS-provoked cell death using quantitative data as mean +/- standard deviation, and statistics. Mechanistically, tartrate prevented DJ-1 aggregation via occupying the P-i-binding site. These findings revealed an unexpected physiological role of tartrate in the maintenance of DJ-1 function, and thus, a potential use as an inhibitor of DJ-1 aggregation. (C) 2017 Elsevier B.V. All rights reserved.
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