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Aberrant single-minded homolog 1 methylation as a potential biomarker for cervical cancer

Title
Aberrant single-minded homolog 1 methylation as a potential biomarker for cervical cancer
Authors
Kim, Hyun-JungKim, Chan YoungJin, JinghuiBae, Moon KyoungKim, Yun HeeJu, WoongKim, Yun HwanKim, Seung Cheol
Ewha Authors
김승철주웅김윤환
SCOPUS Author ID
김승철scopus; 주웅scopus; 김윤환scopus
Issue Date
2018
Journal Title
DIAGNOSTIC CYTOPATHOLOGY
ISSN
8755-1039JCR Link1097-0339JCR Link
Citation
vol. 46, no. 1, pp. 15 - 21
Keywords
cervical cancercirculating cell-free DNAdiagnostic biomarkerDNA methylationsingle-minded homolog 1(SIM1)
Publisher
WILEY
Indexed
SCIE; SCOPUS WOS scopus
Abstract
BackgroundThe aim of this study is to evaluate the possibility of using the methylation status of single-minded homolog 1 (SIM1) as a diagnostic biomarker for cervical cancer. MethodsAll the patient and normal specimens including the normal cervix (n=10), cervical cancer tissues (n=45), blood (n=45), and cervical brush specimens (n=110) were retrospectively obtained. Quantitative methylation-specific PCR was performed to detect SIM1 methylation in primary tumors, cervical brush specimens, and plasma circulating cell-free DNA (ccfDNA). SIM1 expression was detected by western blot analysis. ResultsWe found that SIM1 was highly methylated in the majority of the cervical cancer tissues that we tested, but not in any of the normal tissues. Hypermethylation of SIM1 led to a pronounced reduction in SIM1 expression in cervical cancer tissues compared with normal cervix. SIM1 methylation status on cervical brush specimens also distinguished cervical cancer from normal, cervical intraepithelial neoplasia (CIN) 1 and 2. The degree of SIM1 methylation was significantly associated with the severity of the disease (P-trend<.0001). We also investigated the possibility of detecting methylated SIM1 in plasma ccfDNA from cervical cancer patients. Methylated SIM1 was detected in 36.6% (15/41) of ccfDNA samples, and concordance rate with the matched cancer tissues was 41.5% (17/41) with sensitivity 38.5% and specificity 100%. ConclusionThis study has shown that SIM1 is frequently hypermethylated in cervical cancer, compared with normal cervix tissue, CIN1 and 2 samples, suggesting that the methylation status of SIM1 could be a potential diagnostic biomarker for cervical cancer.
DOI
10.1002/dc.23838
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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