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One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains

Title
One-step fermentative production of aromatic polyesters from glucose by metabolically engineered Escherichia coli strains
Authors
Yang, Jung EunPark, Si JaeKim, Won JunKim, Hyeong JunKim, Bumjoon J.Lee, HyukShin, JihoonLee, Sang Yup
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2018
Journal Title
NATURE COMMUNICATIONS
ISSN
2041-1723JCR Link
Citation
vol. 9
Publisher
NATURE PUBLISHING GROUP
Indexed
SCI; SCIE; SCOPUS WOS
Abstract
Aromatic polyesters are widely used plastics currently produced from petroleum. Here we engineer Escherichia coli strains for the production of aromatic polyesters from glucose by one-step fermentation. When the Clostridium difficile isocaprenoyl-CoA: 2-hydroxyisocaproate CoA-transferase (HadA) and evolved polyhydroxyalkanoate (PHA) synthase genes are overexpressed in a D-phenyllactate-producing strain, poly(52.3 mol% 3-hydroxybutyrate (3HB)-co-47.7 mol% D-phenyllactate) can be produced from glucose and sodium 3HB. Also, various poly(3HB-co-D-phenyllactate) polymers having 11.0, 15.8, 20.0, 70.8, and 84.5 mol% of D-phenyllactate are produced from glucose as a sole carbon source by additional expression of Ralstonia eutropha beta-ketothiolase (phaA) and reductase (phaB) genes. Fed-batch culture of this engineered strain produces 13.9 g l(-1) of poly(61.9 mol% 3HB-co-38.1 mol% D-phenyllactate). Furthermore, different aromatic polyesters containing D-mandelate and D-3-hydroxy-3-phenylpropionate are produced from glucose when feeding the corresponding monomers. The engineered bacterial system will be useful for one-step fermentative production of aromatic polyesters from renewable resources.
DOI
10.1038/s41467-017-02498-w
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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